江苏省域草莓灰霉病菌种群多药剂抗性检测  

Multidrug Resistance Detection of Strawberry Gray Mold Strains in Jiangsu Province

作　　者：肖婷[1] 钱荣明 张富荣 周斌 吴惠秋 季森 宋俊贤 庄义庆[1] 杨敬辉[1] XIAO Ting;QIAN Rongming;ZHANG Furong;ZHOU Bin;WU Huiqiu;JI Sen;SONG Junxian;ZHUANG Yiqing;YANG Jinghui(Zhenjiang Institute of Agricultural Sciences of Jiangsu Hilly Area,Jurong,Jiangsu 212400;Plant Protection and Inspection Station of Lishui District in Nanjing,Nanjing 211000;Plant Protection and Inspection Station of Tongshan District in Xuzhou City,Jiangsu Province,Xuzhou,Jiangsu 221000;Kunshan Cultivated Land Quality and Plant Protection Station,Kunshan,Jiangsu 215300;Plant Protection and Inspection Station of Jiangyan District in Taizhou City,Taizhou,Jiangsu 225500;Wuxi Agricultural Technology Extension Station,Wuxi,Jiangsu 214000;Agricultural and Rural Comprehensive Management Service Center,Xinbei District in Changzhou,Changzhou,Jiangsu 213000)

机构地区：[1]江苏丘陵地区镇江农业科学研究所,江苏句容212400 [2]南京市溧水区植保植检站,南京211000 [3]徐州市铜山区植保植检站,江苏徐州221000 [4]昆山市耕地质量与植物保护站,江苏昆山215300 [5]泰州市姜堰区植保植检站,江苏泰州225500 [6]无锡市农业技术推广总站,江苏无锡214000 [7]常州市新北区农业农村综合管理服务中心,江苏常州213000

出　　处：《中国农学通报》2022年第14期110-117,共8页Chinese Agricultural Science Bulletin

基　　金：江苏省“333工程”资助项目“省域草莓灰霉病菌多药剂抗性检测及机理研究”(BRA2020167)。

摘　　要：本研究旨在阐明江苏省草莓主产区草莓灰霉病菌种群对常用杀菌剂的敏感性。采用区分剂量法测定了2019和2020年采集的236株草莓灰霉病菌(Botrytis cinerea)对13种杀菌剂的抗性,并对菌株抗性频率和多药剂抗性进行了分析。结果表明:所有灰霉菌株对咯菌腈(fludioxonil,Flu)表现敏感,而对多菌灵(carbendazim,Car)、吡唑醚菌酯(pyraclostrobin,Pyra)、扑海因(iprodione,Ipr)、腐霉利(procymidone,Pro)、嘧霉胺(pyrimethanil,Pyr)产生了较高的抗药性,其抗性频率分别为58.05%、55.01%、51.44%、45.80%、33.94%;灰霉病菌种群在地区间对不同药剂的抗药性水平不同,溧水、昆山、姜堰、铜山田间以抗性菌株为优势种群,无锡和常州新北地区以敏感菌株为主;本研究在区分计量浓度下,根据各药剂抑菌率分布状况,将供试药剂分为敏感药剂、敏感性下降药剂和抗性药剂3种类型;本研究共检测到9种抗药性类型:Pyra^(R)(7.32%)、Car^(R)(6.50%)、Pyr^(R)(0.81%)、Car^(R) Pyr^(R)(3.25%)、Car^(R) Pyra^(R)(14.63%)、Car^(R) Bos^(R)(1.63%)、Car^(R) Pyra^(R) Pyr^(R)(23.58%)、Car^(R) Bos^(R) Pyra^(R)(7.31%)、Car^(R) Bos^(R) Pyra^(R) Pyr^(R)(34.15%)。江苏省草莓灰霉病菌种群对多菌灵和吡唑醚菌酯基本丧失了敏感性,对扑海因、腐霉利、啶酰菌胺、戊唑醇等敏感性下降,多药剂抗药性种群以Car^(R) Pyra^(R) Pyr^(R)和Car^(R) Bos^(R) Pyra^(R) Pyr^(R)为主,抗性治理措施中推荐使用不同类型药剂交替防治灰霉病。The purpose is to clarify the resistance level of Botrytis cinerea to common fungicides in main strawberry producing areas of Jiangsu Province.The resistance of 236 strains of Botrytis cinerea collected in 2019 and 2020 to 13 fungicides were measured by discriminative dose method,and the resistant frequency and multidrug resistance of the strains were analyzed.The results showed that all strains of Botrytis cinerea were sensitive to fludioxonil(Flu),but resistant to carbendazim(Car),pyraclostrobin(Pyra),iprodione(Ipr),procymidone(Pro),pyrimethanil(Pyr),and their resistant frequencies were 58.05%,55.01%,51.44%,45.80%,and 33.94%,respectively.Strains of Botrytis cinerea had different resistance levels to different fungicides in different regions,the resistant strains were the dominant species in Lishui,Kunshan,Jiangyan and Tongshan,and the sensitive strains were the main species in Wuxi and Changzhou.According to the distribution of inhibitive rate of each fungicide,the tested fungicides were divided into three types:sensitive,sensitive decrease and resistant.A total of 9 resistant types were detected in this study,including PyraR(7.32%),Car^(R)(6.50%),Pyr^(R)(0.81%),Car^(R) Pyr^(R)(3.25%),Car^(R) PyraR(14.63%),Car^(R) BosR(1.63%),Car^(R) PyraR Pyr^(R)(23.58%),Car^(R) BosR PyraR(7.31%),and Car^(R) BosR PyraR Pyr^(R)(34.15%).The Botrytis cinerea strains of strawberry in Jiangsu Province basically have lost sensitivity to carbendazim and pyraclostrobin,and are sensitive decrease to iprodione,procymidone,pyramidamide and tebuconazole.Car^(R) PyraR Pyr^(R) and Car^(R) BosR PyraR Pyr^(R) are the dominant populations.It is recommended to use different types of fungicides to control Botrytis cinerea alternately in the resistance management measures.

关 键 词：草莓 灰霉病菌 杀菌剂 多药剂抗性 江苏省 

分 类 号：S482.2[农业科学—农药学]