人工脑脊液的制备及其神经保护作用研究  

Study on artificial cerebrospinal fluid preparation and its neuroprotective effect

作　　者：冯欣倩 张坤[1] 张迪 胡钦蕊 毛声俊[1] FENG Xinqian;ZHANG Kun;ZHANG Di;HU Qinrui;MAO Shengjun(Sichuan Engineering Laboratory for Plant-Sourced Drug and Sichuan Research Center for Drug Precision Industrial Technology,Key Laboratory of Drug-Targeting and Drug Delivery System of the Education Ministry and Sichuan Province,West China School of Pharmacy,Sichuan University,Chengdu,Sichuan,610041 P.R.China)

机构地区：[1]四川大学华西药学院,靶向药物与释药系统教育部重点实验室,四川省植物来源工程实验室和四川省小分子药物精准化工程技术研究中心,四川成都610041

出　　处：《华西药学杂志》2022年第3期263-266,共4页West China Journal of Pharmaceutical Sciences

摘　　要：目的探讨人工脑脊液对于原代培养的皮层神经元和小脑颗粒细胞的功能维持作用及对损伤神经元的保护作用。方法制备人工脑脊液,并初步考查其稳定性。以氯化钠注射液、乳酸钠林格注射液及醋酸钠林格注射液为阳性对照,采用MTT比色法检测药物对皮层神经元和小脑颗粒细胞存活率的影响,评价不同药物对谷氨酸、过氧化氢及机械划伤所致损伤神经元的保护作用。结果自制人工脑脊液在加速试验条件下稳定性良好。与各阳性对照组比较,其在维持皮层神经元和小脑颗粒细胞存活率方面的效果更佳,对损伤神经元具有更好的保护作用。当人工脑脊液处方中缺乏钙、镁或磷元素时,均会降低细胞活性。结论自制人工脑脊液质量稳定,具有维持神经细胞正常生理功能与保护损伤细胞的作用。OBJECTIVE To investigate the functional maintenance effect of artificial cerebrospinal fluid(ACSF)on primary cultured cortical neurons and cerebellar granule cells and its protective effect on injured neurons.METHODS The ACSF was prepared and the stability trial for this production was carried out.With sodium chloride injection,sodium lactate Ringer’s injection,and sodium acetate Ringer’s injection as positive controls,MTT assay was used to evaluate the functional maintenance effect of ACSF on primary cultured cortical neurons and cerebellar granule cells and the neuroprotective effect on injured neurons caused by glutamate,hydrogen peroxide,or mechanical scratches separately.RESULTS The self-made ACSF had good stability under accelerated conditions.It maintained the function of cortical neurons and cerebellar granule cells and showed the neuroprotective effect on the injured neurons better than the positive controls.The absence of Ca,Mg,or P in the formulation of ACSF would decrease the cell activity.CONCLUSION The self-made ACSF is of stable quality and has the neuroprotective effect of maintaining the normal physiological functions of nerve cells and reducing injury.

关 键 词：人工脑脊液 林格氏液 神经元 神经保护作用 MTT比色法 兴奋性毒性 氧化应激 

分 类 号：R96[医药卫生—药理学]