类风湿性关节炎患者关节滑液CD4^(+)T细胞分化抑制因子2(Id2)表达升高  

Increased expression of inhibitor of differentiation 2(Id2)in CD4^(+)T cells in joint synovial fluid of patients with rheumatoid arthritis

作　　者：孙浩洋 高键 张佩艳 王雁 畅洋 缪金林 朱平 SUN Haoyang;GAO Jian;ZHANG Peiyan;WANG Yan;CHANG Yang;MIAO Jinlin;ZHU Ping(Department of Clinical Immunology,Xijing Hospital,Air Force Medical University,Xi'an 710032,China;National Translational Science Center for Molecular Medicine,Air Force Medical University,Xi'an 710032,China)

机构地区：[1]空军军医大学:西京医院临床免疫科,陕西西安710032 [2]空军军医大学:国家分子医学转化中心,陕西西安710032

出　　处：《细胞与分子免疫学杂志》2022年第4期333-337,共5页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金(81801599);陕西省自然科学基础研究计划(2019ZY-CXPT-03-01)。

摘　　要：目的检测类风湿性关节炎(RA)患者外周血及关节滑液CD4^(+)T细胞亚群的比例及其分化抑制因子2(Id2)的表达,探讨Id2对CD4^(+)T细胞亚群比例的影响。方法收集RA患者51例(含滑液18例)和健康对照者31例,采用流式细胞术测定RA患者外周血、关节滑液和健康对照者外周血中CD4^(+)T细胞、1型辅助T(Th1)细胞和Th17细胞的比例及其核内Id2的表达水平。结果与健康对照组相比,RA患者外周血中CD4^(+)T细胞、Th1细胞及Th17细胞比例及Id2表达水平无明显变化,滑液CD4^(+)T细胞、Th1细胞比例及CD4^(+)T细胞Id2表达水平明显增加,也高于RA患者外周血。CD4^(+)T细胞Id2表达率与血沉(ESR)、C反应蛋白(CRP)及28关节疾病活动度评分(DAS28)无明显相关性,但与γ干扰素(IFN-γ)表达呈正相关。结论RA患者关节滑液中CD4^(+)T细胞富集,其Id2表达促进Th1细胞分化。Objective To investigate the effect of inhibitor of differentiation 2(Id2)on the proportion of CD4^(+)T cells by detecting the proportion of CD4^(+)T cell subsets and Id2 expression in peripheral blood and joint synovial fluid of patients with rheumatoid arthritis(RA).Methods A total of 51 RA patients(including 18 patients providing synovial fluid)and 31 healthy controls(HCs)were enrolled.The proportions of CD4^(+)T cells,Th1 cells,and Th17 cells,and their expression of Id2 in peripheral blood and synovial fluid of RA patients and HCs were detected by flow cytometry.Results Compared with HCs group,the proportions of circulating CD4^(+)T cells,Th1 cells,and Th17 cells and their expression of Id2 in RA patients did not change significantly.The proportions of CD4^(+)T cells and Th1 cells,and Id2 expression in CD4^(+)T cells in synovial fluid of RA patients were significantly higher than those in peripheral blood of RA patients and HCs.The expression rate of Id2 in CD4^(+)T cells was positively correlated with the expression of IFN-γ,but not with erythrocyte sedimentation rate(ESR),C reactive protein(CRP),and Disease Activity Score 28(DAS28).Conclusion CD4^(+)T cells are enriched in RA synovial fluid,and their Id2 expression may promote Th1 cell differentiation.

关 键 词：类风湿性关节炎(RA) 分化抑制因子2(Id2) CD4^(+)T细胞 滑液(SF) 

分 类 号：R593.22[医药卫生—内科学] R392.12[医药卫生—临床医学]