GCM1通过激活Wnt3a/β-catenin信号通路改善绝经后骨质疏松症  

GCM1 improves postmenopausal osteoporosis by activating the Wnt3a/β-catenin signaling pathway

作　　者：谢菲飞 宋立稳[2] 王小英 胡燕芳 谢蕴云 刘文华 谢宝强 XIE Feifei;SONG Liwen;WANG Xiaoying;HU Yanfang;XIE Yunyun;LIU Wenhua;XIE Baoqiang(Department of Endocrinology,Guangdong Provincial People’s Hospital Ganzhou Hospital(Ganzhou Municipal Hospital,Ganzhou 341000;Department of Endocrinology,Weifang People’s Hospital,Weifang 261000,China)

机构地区：[1]广东省人民医院赣州医院(赣州市立医院)内分泌代谢科,江西赣州341000 [2]潍坊市人民医院内分泌代谢科,山东潍坊261000

出　　处：《中国骨质疏松杂志》2022年第6期805-811,共7页Chinese Journal of Osteoporosis

基　　金：江西省卫生计生委科技计划(20194067)。

摘　　要：目的探究GCM1对小鼠胚胎成骨细胞前体细胞(MC3T3-E1)成骨分化及矿化的影响。方法实验设置control组、BMP2诱导组、BMP2+ov-NC组、MP2+ov-GCM1组、BMP2+sh-NC组、BMP2+sh-GCM1组,BMP2用于诱导细胞成骨分化。通过碱性磷酸酶(ALP)染色及茜素红染色检测GCM1对MC3T3-E1成骨分化及矿化的影响;通过Western blot检测成骨分化相关蛋白及Wnt3a/β-catenin蛋白的表达变化。结果GCM1在绝经后骨质疏松症患者血清中低表达。过表达GCM1能够促进BMP2诱导MC3T3-E1的成骨分化和矿化,而抑制GCM1表达则抑制了BMP2诱导MC3T3-E1的成骨分化和矿化。此外,过表达GCM1增加了Wnt3a/β-catenin蛋白表达,激活Wnt3a/β-catenin信号通路,抑制GCM1表达则与上述结果相反。结论GCM1在绝经后骨质疏松症患者血清中低表达,过表达GCM1则可能通过激活Wnt3a/β-catenin信号通路、促进MC3T3-E1成骨分化及矿化,进而改善绝经后骨质疏松症。Objective To explore the effect of GCM1 on osteogenic differentiation and mineralization of mouse embryonic osteoblast progenitor cells(MC3T3-E1).Methods The experiment was divided into blank group,BMP2 induction group,BMP2+ov-NC group,BMP2+ov-GCM1 group,BMP2+sh-NC group and BMP2+sh-GCM1 group.BMP2 was used to induce osteogenic differentiation of cells.Alkaline phosphatase(ALP)staining and alizarin red staining were used to detect the effect of GCM1 on osteogenic differentiation and mineralization of MC3T3-E1.The expression of osteogenic differentiation-related protein and Wnt3a/β-catenin protein was detected by Western blot.Results GCM1 was lowly expressed in the serum of postmenopausal osteoporosis patients.Overexpression of GCM1 could promote BMP2-induced osteogenic differentiation and mineralization of MC3T3-E1,while inhibition of GCM1 expression inhibited BMP2-induced osteogenic differentiation and mineralization of MC3T3-E1.In addition,overexpression of GCM1 increased Wnt3a/β-catenin protein expression and activated Wnt3a/β-catenin signaling pathway,while inhibition of GCM1 expression was contrary to the above result.Conclusion The expression of GCM1 is lowly expressed in the serum of postmenopausal osteoporosis patients.Overexpression of GCM1 can promote osteogenic differentiation and mineralization of MC3T3-E1 by activating Wnt3a/β-catenin signal pathway,which can improve postmenopausal osteoporosis.

关 键 词：绝经后骨质疏松症 GCM1 wnt3a/β-catenin信号通路 成骨分化 矿化 

分 类 号：R34[医药卫生—基础医学]