淫羊藿总黄酮和戊酸雌二醇对骨质疏松症大鼠TGF-β1/Smads信号通路的影响  

The effects of epimedium flavonoids and estradiol valerate on TGF-β1/Smads signal pathway in osteoporotic rats

作　　者：肖平 侯平 林正坚 刘元豪 周钢[2] XIAO Ping;HOU Ping;LIN Zhengjian;LIU Yuanhao;ZHOU Gang(Department of Orthopedics,Haikou Third People’s Hospital,Haikou 571100;Department of Joint Surgery,Hainan Hospital Affiliated to Hainan Medical College,Hainan Provincial People’s Hospital,Haikou 570311)

机构地区：[1]海口市第三人民医院骨科,海南海口571100 [2]海南省人民医院(海南医学院附属海南医院)关节外科,海南海口570311

出　　处：《中国骨质疏松杂志》2022年第6期818-824,共7页Chinese Journal of Osteoporosis

摘　　要：目的研究淫羊藿总黄酮和戊酸雌二醇对骨质疏松症大鼠TGF-β1/Smads信号通路的影响。方法75只雌性SD大鼠随机分为假手术组(Sham组)、模型组(Model组)、淫羊藿总黄酮组(TFE组)、戊酸雌二醇组(E2V组)、淫羊藿总黄酮联合戊酸雌二醇组(TFE+E2V组),行双侧卵巢切除术造模,术后12周开始给予相应的药物干预12周。分别检测血清骨代谢指标,BMD和骨灰重比,骨骼生物力学参数,股骨形态计量学参数,股骨TGF-β1/Smads蛋白和mRNA表达。结果模型组血清Ca、P、OC、PINP,股骨和椎体骨密度、灰重比,股骨最大载荷、最大位移、最大应力、弹性模量,股骨Tb.Ar、Tb.Th、Tb.N,股骨TGF-β1、Smads2蛋白和mRNA表达较Sham组均显著降低(P<0.01),E2V组、TFE组、TFE+E2V组上述指标较模型组均显著增加(P<0.05),TFE+E2V组上述指标较E2V组、TFE组均显著增加(P<0.05)。模型组血清ALP,股骨Tb.Sp、Oc.N较Sham组显著增加(P<0.01),E2V组、TFE组、TFE+E2V组上述指标较模型组均显著降低(P<0.05),TFE+E2V组上述指标较E2V组、TFE组均显著降低(P<0.05)。结论淫羊藿总黄酮联合戊酸雌二醇通过上调TGF-β1、Smad2表达,调节骨质疏松大鼠骨代谢,增加骨量,提高骨密度和骨强度,从而起到抗骨质疏松作用。Objective To study the effects of total flavonoids of epimedium and estradiol valerate on TGF-β1/Smads signaling pathway in osteoporotic rats.Methods Seventy-five female SD rats were randomly divided into sham operation group(Sham group),model group(Model group),epimedium total flavonoids group(TFE group),estradiol valerate group(E2V group),and epimedium total flavonoids combined with estradiol valerate group(TFE+E2V group),Osteoporotic rat model was created with bilateral ovariectomy,Corresponding drug intervention was performed for 12 weeks after 12-week of operation.Serum bone metabolism indexes,BMD and bone ash weight ratio,bone biomechanical parameters,femoral morphometric parameters,femoral TGF-β1/Smads protein and mRNA expression were detected.Results Serum Ca,P,OC,PINP,BMD of the femur and vertebra,ash-to-weight ratio,femur maximum load,maximum displacement,maximum stress,elastic modulus,Tb.Ar,Tb.Th,and Tb.N of the femur,and the protein and mRNA expression of TGF-β1 and Smads2 in Model group were significantly lower than those in the Sham group(P<0.01).The above indicators in the E2V group,TFE group,and TFE+E2V group were significantly higher than those in the Model group(P<0.05),and the above indicators in the TFE+E2V group were significantly higher than those in the E2V and TFE groups(P<0.05).Serum ALP,femoral Tb.Sp and Oc.N in the Model group were significantly higher than those in the Sham group(P<0.01).The above indicators in the E2V group,TFE group,and TFE+E2V group were significantly lower than thsoe in the Model group(P<0.05).The above indicators in the TFE+E2V group were significantly lower than those in the E2V and TFE groups(P<0.05).Conclusion Epimedium total flavonoids combined with estradiol valerate up-regulates the expression of TGF-β1 and Smad2 to regulate bone metabolism,increases bone mass,and improves BMD and strength in osteoporotic rats,thereby playing an anti-osteoporotic effect.

关 键 词：淫羊藿总黄酮 雌二醇 骨质疏松 转化生长因子Β-1 SMADS 

分 类 号：R284.1[医药卫生—中药学] R-332[医药卫生—中医学]