健骨胶囊对成骨样细胞MC3T3-E1增殖及分化作用机制  

Effect and mechanism of Jiangu capsule on the proliferation and differentiation of osteoblast-like cell MC3T3-E1

作　　者：贾玉岩 周振薇 王旭凯[3] 杨宇安 刘茜[3] 幺宝金 冷向阳 JIA Yuyan;ZHOU Zhenwei;WANG Xukai;YANG Yu’an;LIU Qian;YAO Baojin;LENG Xiangyang(College of Traditional Chinese Medicine,Changchun University of Chinese Medicine,Changchun 130117;Jilin Ginseng Academy,Changchun University of Chinese Medicine,Changchun 130117;Department of Orthopedic,the Affiliated Hospital of Changchun University of Chinese Medicine,Changchun 130021;College of Continuing Education,Changchun University of Chinese Medicine,Changchun 130021,China)

机构地区：[1]长春中医药大学中医学院,吉林长春130117 [2]长春中医药大学吉林省人参科学研究院,吉林长春130117 [3]长春中医药大学附属医院骨科,吉林长春130021 [4]长春中医药大学附属医院继续教育学院,吉林长春130117

出　　处：《中国骨质疏松杂志》2022年第6期863-867,880,共6页Chinese Journal of Osteoporosis

基　　金：吉林省中医骨病临床医学研究中心项目(20180623048TC)。

摘　　要：目的探讨国医大师刘柏龄“健骨胶囊”对MC3T3-E1成骨细胞分化及增殖的影响。方法制备健骨胶囊水提物,采用CCK-8法和细胞迁移实验检测健骨胶囊提取物对MC3T3-E1细胞增殖和细胞迁移的影响;茜素红染色检测MC3T3-E1细胞的矿化能力;实时荧光定量PCR检测成骨分化基因Runx2、OCN、OPN、Col1a1、ALP、Bcl2、RASSF1A等mRNA表达水平;蛋白质印迹法Western blot检测Col1a1、Bcl2的蛋白表达水平。结果通过实验结果比对得出,健骨胶囊提取物能促进MC3T3-E1细胞增殖、使细胞迁移率提高;同时健骨胶囊提取物组能明显提高MC3T3-E1细胞钙化能力(P<0.01),促进Runx2、OCN、OPN、Col1a1、ALP、Bcl2的mRNA表达(P<0.05),上调Col1a1、Bcl2蛋白量的表达。结论健骨胶囊能促进成骨细胞MC3T3-E1的增殖及细胞迁移能力,并通过上调成骨基因的表达水平如Runx2、OCN、OPN、Col1a1、ALP、Bcl2等,提高MC3T3-E1细胞的成骨能力。Objective To investigate the effect of Jiangu capsule by Liu Bailing,a master of Chinese medicine,on the differentiation and proliferation of MC3T3-E1 osteoblasts.Methods The water extract of Jiangu capsule was lyophilized.CCK-8 method and cell migration test were used to detect the effect of the extract on the proliferation and migration of MC3T3-E1 cells.The mineralization capability of MC3T3-E1 cells was detected with Alizarin red staining.The mRNA levels of osteogenic differentiation genes Runx2,OCN,OPN,Col1a1,ALP,and Bcl2 were detected using real-time quantitative PCR.The protein expression levels of Col1a1 and Bcl2 were detected with Western blotting.Results The experimental result showed that the extract of Jiangu capsule promoted the proliferation of MC3T3-E1 cells and increased cell mobility.At the same time,the extract of Jiangu capsule significantly improved the calcification ability of MC3T3-E1 cells(P<0.01),promoted the mRNA expression of Runx2,OCN,OPN,Col1a1,ALP,and Bcl2(P<0.05),and promoted the expression of Col1a1 and Bcl2 proteins.Conclusion Jiangu capsule promotes the proliferation and migration of MC3T3-E1 osteoblasts and improves the osteogenic capability of MC3T3-E1 cells by up-regulating the expression levels of osteogenic genes such as Runx2,OCN,OPN,Col1a1,ALP,and Bcl2.

关 键 词：健骨胶囊 中医中药 MC3T3-E1细胞 细胞增殖 成骨分化 

分 类 号：R965.2[医药卫生—药理学]