高压液相色谱法同时测定泌感合剂中6种黄酮类成分研究  

Simultaneous Determination of Six Flavonoids in Migan Mixture by HPLC

作　　者：彭敏 符映均 童晓东 姜晓燕 杨水英 刘忠美 倪红辉 张硕 PENG Min;FU Yingjun;TONG Xiaodong;JIANG Xiaoyan;YANG Shuiying;LIU Zhongmei;NI Honghui;ZHANG Shuo(Nantong Hospital of Traditional Chinese Medicine,Jiangsu Province,Nantong 226000,China)

机构地区：[1]江苏省南通市中医院,南通226000

出　　处：《世界中医药》2022年第10期1368-1370,1376,共4页World Chinese Medicine

基　　金：江苏省卫生健康委项目2021年度医学科研项目(Z2021081);南通市中医医疗联盟课题(TZYK201811,TZYK201803)。

摘　　要：目的:建立高压液相色谱法(HPLC)同时分析并测定泌感合剂中落新妇苷、野黄芩苷、野黄芩素、木犀草素、芹菜素、异鼠李素的含量。方法:采用Agilent ZORBAX SB-C18色谱柱(4.6×250 mm,5μm);流动相:甲醇(A)-0.4%磷酸水(B),梯度洗脱;检测波长320 nm;流速1.0 mL/min;柱温23℃。结果:落新妇苷、野黄芩苷、野黄芩素、木犀草素、芹菜素、异鼠李素分别在0.144~1.44μg(r=0.9999)、0.196~1.96μg(r=0.9997)、0.076~0.76μg(r=0.9993)、0.034~0.34μg(r=0.9993)、0.032~0.32μg(r=0.9998)、0.026~0.26μg(r=0.9995)范围内进样量与峰面积线性关系良好;平均回收率在分别为95.6%、96.3%、94.5%、92.3%、97.8%、95.2%;相对标准偏差(RSD)分别为1.7%、1.5%、2.2%、1.2%、2.0%、1.6%。结论:该方法操作简单、稳定、可重复,可用于同时分析测定泌感合剂中6种黄酮类成分的含量。Objective:To develop a method for simultaneous determination of astilbin,scutellarin,scutellarein,luteolin,apigenin,and isorhamnetin in Migan Mixture by high-performance liquid chromatography(HPLC).Methods:Separation was performed on an Agilent ZORBAX SB-C18 column(4.6 mm×250 mm,5μm)at 23℃.The mobile phases were methanol(A)-0.4%phosphoric acid(B)with gradient elution.The flow rate was 1.0 mL·min-1 and the detection wavelength was 320 nm.Results:Astilbin,scutellarin,scutellarein,luteolin,apigenin,and isorhamnetin showed good linearity between the injection volume and the peak area at 0.144~1.44μg(r=0.9999),0.196~1.96μg(r=0.9997),0.076~0.76μg(r=0.9993),0.034~0.34μg(r=0.9993),0.032~0.32μg(r=0.9998),and 0.026~0.26μg(r=0.9995),respectively.The average recoveries were 95.6%,96.3%,94.5%,92.3%,97.8%,and 95.2%,with RSDs of 1.7%,1.5%,2.2%,1.2%,2.0%,and 1.6%,respectively.Conclusion:This method is simple,stable,and repeatable,which can be used to determine the content of six flavonoids in Migan Mixture by HPLC.

关 键 词：泌感合剂 落新妇苷 野黄芩苷 野黄芩素 木犀草素 芹菜素 异鼠李素 高压液相色谱法 含量测定 

分 类 号：R285.5[医药卫生—中药学]