基于网络药理学探讨侗药血水草活性成分抗肿瘤的作用机制  

Study on Antitumor Mechanism of Eomecn Chionantha Hance Based on Network Pharmacology

作　　者：梁沛 胡芷茵 杨翠玉 周小强 周永涛 周群 LIANG Pei;HU Zhiyin;YANG Cuiyu(Hu’nan University of Medicine,Huaihua City,Hu’nan Province 418000)

机构地区：[1]湖南医药学院,湖南省怀化市418000

出　　处：《医学理论与实践》2022年第12期1985-1989,共5页The Journal of Medical Theory and Practice

基　　金：湖南省自然科学基金(2019JJ50424);湖南省大学生创新创业训练项目(20182551233)。

摘　　要：目的:运用网络药理学的技术和方法,探讨与分析侗药血水草生物碱类活性成分血根碱、原阿片碱、别隐品碱、白屈菜红碱多靶点、多通路的抗肿瘤作用机制。方法:运用TCMSP数据库验证血水草生物碱类活性成分;利用PubChem、SwissTargetPrediction、GeneCards数据库预测和筛选4种活性成分的相关肿瘤靶点基因,经Venny软件获得交集靶点基因;使用STRING数据库平台构建4种化合物的PPI;运用Cytoscape3.7.1软件经Network tools网络拓扑参数分析,筛选出Hub基因,并构建“药物成分—关键靶点—疾病”三者的相互关系网络;使用DAVID 6.8数据库对Hub基因进行GO生物功能和KEGG富集分析。结果:本研究共收集到成分相关靶点239个,疾病相关靶点9910个,其中两者的交集靶点199个,经拓扑参数得到Hub基因分别是HSP90AA1、MAPK1、MTOR、APP、SRC、SIRT1等;对Hub基因进行GO生物功能和KEGG通路富集分析得到多条肿瘤相关通路包括癌症通路、神经营养素信号通路等。结论:侗药血水草生物碱类活性成分抗肿瘤的作用表现出多靶点、多通路的特征,主要通过HSP90AA1、MAPK1、MTOR、APP、SRC、SIRT1等关键靶点调控肿瘤的发生发展,与多条信号通路存在着密切关系,为侗药血水草生物碱活性成分抗肿瘤的作用机制研究提供了新的思路与依据。Objective:The techniques and methods of network pharmacology were used to explore and analyze the anti-tumor mechanism of sanguinarine,protopine,allocyptopine and chelerythrine with multi-targets and multi-pathways.Methods:TCMSP database was used to verify the alkaloid active components,PubChem,SwissTargetPrediction and GeneCards databases were used to predict and screen the related tumor target genes of the four active components,and the intersection target genes were obtained by Venny software.The PPI maps of the four compounds were constructed by STRING database platform.Using Cytoscape3.7.1 software to analyze the topological parameters of Networktools network,the Hub gene was screened,and the relationship network diagram of“drug ingredient-key target-disease”was constructed,and the GO biological function and KEGG enrichment of Hub gene were analyzed by DAVID6.8 database.Results:A total of 239 component-related targets and 9910 disease-related targets were collected in this study,of which the two intersected targets were 199.Through topological parameters,Hub genes were obtained as HSP90AA1,MAPK1,MTOR,APP,SRC,SIRT1,etc.Multiple tumor-related pathways were obtained by GO biological function and KEGG pathway enrichment analysis of Hub gene,including pathways in cancer,neurotrophin signal pathway and so on.Conclusion:The anti-tumor effect of alkaloids from Herba Euphorbiae shows the characteristics of multi-targets and multi-pathways,which mainly regulates the occurrence and development of tumors through key targets such as HSP90AA1,MAPK1,MTOR,APP,SRC and SIRT1,which is closely related to multiple signal pathways,which provides a new idea and basis for the study of the anti-tumor mechanism of alkaloids from Eomecn chionantha Hance.

关 键 词：网络药理学 血水草活性成分 肿瘤 机制研究 

分 类 号：R9[医药卫生—药学]