柑橘次级代谢产物β-蒎烯对柑橘溃疡病菌的生长抑制作用与机理初探  

Citrus metabolite β-pinene exerts a dual function on citrus canker pathogen Xanthomonas citri subsp. citri

作　　者：叶子怡 范晓静[1] 卓涛[1] 邹华松[1] 户勋[1] YE Ziyi;FAN Xiaojing;ZHUO Tao;ZOU Huasong;HU Xun(College of Plant Protection,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China)

机构地区：[1]福建农林大学植物保护学院,福建福州350002

出　　处：《武夷科学》2022年第1期25-30,共6页Wuyi Science Journal

基　　金：福建省教育厅项目(JT180159);福建省自然科学基金(2019J01372)。

摘　　要：通过体内外生长曲线和表型观察试验,分析不同浓度β-蒎烯对柑橘溃疡病菌(Xcc 29-1)的生长抑制作用和柑橘溃疡病症状形成的影响。利用β-葡萄糖苷酸酶基因的定性检测和实时荧光定量PCR分析不同浓度β-蒎烯对Xcc 29-1Ⅲ型分泌系统相关基因hrp G的诱导作用。结果表明:随着浓度的升高,β-蒎烯对Xcc 29-1的生长抑制增强,0.2μg·mL^(-1)的β-蒎烯能显著抑制溃疡病菌的生长繁殖,培养基中添加1μg·mL^(-1)β-蒎烯时,Xcc 29-1几乎不能生长。同时,0.1μg·mL^(-1)的β-蒎烯能有效在体内外诱导Ⅲ型分泌系统相关基因hrp G、hrp X、hrc V和hrp D6的转录表达。β-蒎烯作为柑橘的次级代谢产物可抑制Xcc 29-1的生长繁殖,抑菌作用的大小与其浓度正相关。与此同时,β-蒎烯能诱导Ⅲ型分泌系统相关基因的表达。说明β-蒎烯抑制的不是菌物的致病力,而是菌物的繁殖。The influence on the growth of Xanthomonas citri subsp.citri(Xcc 29-1)was examined by cell growth curves.In addition,to evaluate the role ofβ-pinene on canker symptom development,citrus leaves were inoculated with Xcc 29-1 suspension containingβ-pinene.Promoter activity test and real-time quantitative PCR assays were conducted to examine the induction ofβ-pinene on hrp G genes.The results show that,0.2μg·mL^(-1)β-pinene inhibited the Xcc 29-1 growth,while Xcc 29-1 cannot grow when the concentration ofβ-pinene is up to 1μg·mL^(-1).Further,0.1μg·mL^(-1)β-pinene induced the expression of hrp G,hrp X,hrc V,and hrp D6.β-pinene is one of the secondary metabolites of citrus.It can be used by citrus to inhibit Xcc 29-1,while it can be used by Xcc 29-1 to induce the expression of hrp genes as a plant signal.Hence,it shows thatβ-pinene influences the multiplication of Xcc 29-1,not the pathogenicity.

关 键 词：柑橘溃疡病 Β-蒎烯 HRP基因 诱导表达 抑制生长 

分 类 号：S432[农业科学—植物病理学]