复合树脂及复合体对成骨细胞毒性及成骨向分化的影响  

In vitro cytotoxicity of composite resin and compomer and effect on osteogenic differentiation of osteoblasts

作　　者：张静怡 李丹薇[1] 孙宇 雷雅燕[1] 刘涛[1] 龚瑜[1] Zhang Jingyi;Li Danwei;Sun Yu;Lei Yayan;Liu Tao;Gong Yu(Dept.of Cariology and Endodontics,The Affiliated Stomatological Hospital of Kunming Medical University,Kunming 650106,China;Comfort Treatment Center,Kunming Blue Orange Stomatological Hospital,Kunming 650228,China)

机构地区：[1]昆明医科大学附属口腔医院牙体牙髓病科,昆明650106 [2]昆明蓝橙口腔医院舒适化治疗中心,昆明650228

出　　处：《国际口腔医学杂志》2022年第4期412-419,共8页International Journal of Stomatology

基　　金：云南省教育厅科学研究基金(2018JS234)。

摘　　要：目的通过观察口腔修复材料复合树脂、复合体对成骨细胞毒性及成骨向分化的影响,探索修复牙根缺损的新方法。方法建立小鼠成骨细胞前体细胞(MC3T3-E1)体外成骨诱导实验模型,通过扫描电子显微镜(SEM)、CCK-8试剂盒(cell counting kit-8)检测、细胞凋亡和细胞周期检测、划痕实验、碱性磷酸酶(ALP)活性检测、茜素红染色和实时定量聚合酶链式反应(qRT-PCR)比较两种材料对MC3T3-E1细胞的生物学影响。结果MC3T3-E1细胞在两种材料上黏附良好;复合树脂浸提液对MC3T3-E1表现出稍高的细胞毒性,但其可促进细胞迁移,上调骨钙素(OCN)、骨桥蛋白(OPN)、runt相关转录因子2(RUNX2)和Ⅰ型胶原蛋白(COL-1)mRNA的表达,而复合体浸提液显著抑制了成骨相关基因的表达(P<0.05)。结论轻微的细胞毒性并不会显著影响成骨细胞黏附生长于复合树脂和复合体材料上,两种生物材料不会影响成骨细胞的正常分化,初步体现了骨性替代材料的基本性能。Objective To explore a new method for repairing root defects by observing the osteoblast toxicity of composite resin and compomer,as well as their effects on osteogenic differentiation.Methods An experimental model of MC3T3-E1 was established for osteogenesis induction in vitro.Scanning electron microscope(SEM),cell counting kit-8(CCK-8)detection,apoptosis and cell cycle detection,scratch experiment,alkaline phosphatase(ALP)activity detection,Alizarin red staining,and quantitative real-time polymerase chain reaction(qRT-PCR)were performed to compare the biological effects of the two materials on MC3T3-E1 cells.Results The MC3T3-E1 cells adhered to the two kinds of materials well.The composite resin extract showed slightly higher cytotoxicity to MC3T3-E1 but could promote cell migration and upregulate osteocalcin(OCN),osteopontin(OPN),runt-related transcription factor 2(RUNX2),and collagen 1(COL-1)mRNA expression.The compomer extract significantly inhibited the expression of osteogenic differentiation-related genes(P<0.05).Conclusion The slight cytotoxicity of the composite resin and compomer did not considerably affect the adhesion,growth,and normal differentiation of osteoblasts;thus,both materials have potential as bone replacement materials.

关 键 词：复合树脂 复合体 成骨细胞 细胞毒性 成骨向分化 

分 类 号：Q291[生物学—细胞生物学]