外周血T淋巴细胞亚群及血清可溶性E-选择素在慢性光化性皮炎患者发病中的研究  

Correlation Between Peripheral Blood T Lymphocyte Subsets and Serum E-election Levels in Patients with Chronic Actinic Dermatitis

作　　者：韩洋洋 杨丹琦 王剑锋 高晓莉 冯冬梅[2] 周向昭[2] Han Yangyang;Yang Danqi;Wang Jianfeng;Gao Xiaoli;Feng Dongmei;Zhou Xiangzhao(Hebei North University,Zhangjiakou 075000,China;the First Affiliated of Hospital Hebei North University,Zhangjiakou 075000,China)

机构地区：[1]河北北方学院,河北张家口075000 [2]河北北方学院附属第一医院,河北张家口075000

出　　处：《中国中西医结合皮肤性病学杂志》2022年第3期219-221,共3页Chinese Journal of Dermatovenereology of Integrated Traditional and Western Medicine

基　　金：河北省科技厅重点研发计划自筹项目(编号:182777219)。

摘　　要：目的检测慢性光化性皮炎(CAD)患者外周血T细胞亚群及血清E-选择素的表达,初步探讨其在CAD发病中的作用。方法选取我院诊断为CAD患者48例(观察组),取患者晨起空腹静脉血,采用流式细胞技术和亲合素-生物素过氧化物酶复合物酶联免疫吸附测定(ABC-ELISA)双夹心抗体法检测CAD患者外周血T淋巴细胞亚群和血清E-选择素表达水平,并设同期来院体检正常人30例,作为对照组,进行对比分析。结果观察组CD3^(+)CD4^(+)、CD3^(+)CD4^(+)/CD3^(+)CD8^(+)明显低于对照组,经比较差异有统计学意义(P<0.05)。观察组CD3^(+)CD8^(+)明显高于对照组CD3^(+)CD8^(+),经比较差异有统计学意义(P<0.05)。观察组CD3^(+)与对照组CD3^(+)相比,经比较差异无统计学意义(P>0.05)。观察组血清E-选择素明显高于对照血清E-选择素,经比较差异有统计学意义(P<0.05)。结论CAD患者T淋巴细胞亚群分布异常,CD8^(+)T淋巴细胞可能参与了CAD的迟发型超敏反应。E-选择素参与了CAD的炎性反应,是其病情变化的重要指标。Objective In order to detect the expression of peripheral blood T cell subsets and serum E-selectin in patients with chronic actinic dermatitis(CAD)and to explore its role in the pathogenesis of CAD.Methods Forty-eight patients with CAD diagnosed in our hospital were selected as observation group.Fasting venous blood was taken from patients with CAD in the morning.Flow cytometry and avidin biotin complex-enzyme-linked immunosorbent assay(ABC-ELISA)double sandwich antibody method were used to detect T lymphocyte subsets and serum E-selectin expression in patients with CAD in observation group.In the same period,30 normal subjects were selected as control group.Results The levels of CD3^(+)CD4^(+)and CD3^(+)CD4^(+)/CD3^(+)CD8^(+)in the observation group were significantly lower than those in the control group(P<0.05).The CD3^(+)CD8^(+)in the observation group was significantly higher than that in the control group(P<0.05).There were significant differences in CD3^(+)CD8^(+)between the two groups.There was no significant difference in CD3^(+)between the observation group and the control group(P>0.05).The average level of serum E-selectin in the observation group was significantly higher than that in the control group,and the differences were statistically significant(P<0.05).Conclusion The distribution of T lymphocyte subsets in patients with CAD is abnormal,and CD8^(+)T lymphocytes may be involved in the delayed type hypersensitivity of CAD.E-selectin is involved in the inflammatory response of CAD,and it is an important indicator of the disease changes.

关 键 词：慢性光化性皮炎 T淋巴细胞亚群 E-选择素 

分 类 号：R758.14[医药卫生—皮肤病学与性病学]