大豆转录因子GmWRKY32基因的生物信息学及表达分析  

Bioinformatics and Expression Analysis of Soybean Transcription Factor GmWRKY 32 Gene

作　　者：赵琳 赵婕 孙永媛[2] 曹金锋[2] 刘建凤[1] Zhao Lin;Zhao Jie;Sun Yongyuan;Cao Jinfeng;Liu Jianfeng(College of Life Science,Hebei University,Baoding.071002;Hebei Key Laboratoryof Crop Salt-alkali Stress Tolerance Evaluation and Genetic Improvement,Cangzhou Academy of Agriculture and Forestry Sciences,Cangzhou061001)

机构地区：[1]河北大学生命科学学院,保定071002 [2]沧州市农林科学院河北省农作物耐盐碱评价与遗传改良重点实验室,沧州061001

出　　处：《分子植物育种》2022年第10期3188-3194,共7页Molecular Plant Breeding

基　　金：河北大学在读研究生创新能力培养资助项目(hbu2020ss036)资助。

摘　　要：WRKY类转录因子参与植物生长发育及逆境胁迫的响应。本研究利用大豆基因组数据库中的WRKY序列,克隆了大豆GmWRKY32基因开放阅读框(ORF)为903 bp,其编码300个氨基酸。生物信息学分析发现GmWRKY32蛋白含有1个高度保守的WRKY结构域,且具有CX_(7)CX_(22)HXXC锌脂结构,属于WRKY基因家族的第Ⅲ类。与野生大豆Gs WRKY70、木豆CcWRKY70和山葡萄VaWRKY70蛋白的同源性分别为85%、79%和72%。此外GmWRKY32亲水性较弱疏水性较强,属于不可溶性蛋白,无信号肽和跨膜结构域。进一步分析该基因在不同逆境胁迫及激素诱导下的表达模式,研究结果表明在PEG、高盐胁迫和激素JA诱导处理12 h后,GmWRKY32表达量较对照分别增加了4.5、4.2和3.2倍;在激素ABA诱导处理24 h后,GmWRKY32表达量较对照增加了6倍。推测其与大豆抵御非生物胁迫密切相关,并为大豆抗性研究和分子育种提供科学依据为进一步研究大豆WRKY的家族结构和功能奠定基础,并为大豆抗性研究和分子育种提供科学依据。WRKY transcr iption factors are very important transcriptional regulators in the process of plant response to plant growth and abiotic stresses.In this study,the open reading frame(ORF)of soybean Gm WRKY32 gene was 903 bp,encoding 300 amino acids,using WRKY sequences from soybean genome database.Bioinformatics analysis revealed that the GmWRKY32 protein contained a highly conserved WRKY domain and belonged to GroupⅢof the WRKY gene family.The homology with Glycine soja Gs WRKY70,Cajanus cajan CcWRKY70 and Vigna angularis VaWRKY70 proteins was 85%,79%and 72%,respectively.In addition,GmWRKY32 hydrophilic weak hydrophobic strong,is an insoluble protein,no signal peptide and transmembrane domain.Further analysis of the gene expression pattern under different stress and hormone induction showed that after 12 h of treatment with PEG,high salt stress and JA,the expression of Gm WRKY32 was increased by 4.5,4.2 and 3.2 times as much as that in the control,respectively.After 24 h of ABA induction,the expression level of Gm WRKY32 was increased6-fold compared with that of the control.Conjecture that it is closely related to the resistance of soybean to abiotic stress which would lay a foundation for the family structure and functional research of soybean WRKY transcription factors,and will provide scientific basis for research of soybean resistance and molecular breeding.

关 键 词：大豆(Glycine max.L.) GmWRKY32 生物信息学分析 非生物胁迫 

分 类 号：S565.1[农业科学—作物学]