白花真樱组蛋白乙酰转移酶hat8基因的克隆和表达分析  

Cloning and Expression Analysis of hat8 Gene in Prunus lannesiana cv.Multiplex

作　　者：李文[1] 蒋冬月[2] 周孟凡 胡勇 潘贺洁 胡琼芳[1] 宋帅杰 付涛[1] 何月秋[1] 王国良[4] 王志龙[1] Li Wen;Jiang Dongyue;Zhou Mengfan;Hu Yong;Pan Hejie;Hu Qiongfang;Song Shuaijie;FuTao;He Yueqiu;Wang Guoliang;Wang Zhilong(City College of Vocational Technology Utilization of Ningbo,Ningbo,315199;Zhejiang Academy of Forestry,Hangzhou,310023;HuilüLand-scape Construction Development Co.,Ltd.,Ningbo,315100;College of Biological Environmental Sciences,Zhejiang Wanli University,Ningbo,315100)

机构地区：[1]宁波城市职业技术学院,宁波315199 [2]浙江省林业科学研究院,杭州210023 [3]汇绿园林建设发展有限公司,宁波315100 [4]浙江万里学院生物与环境学院,宁波315100

出　　处：《分子植物育种》2022年第10期3195-3202,共8页Molecular Plant Breeding

基　　金：浙江省省院合作林业科技项目(2021SY10);宁波市自然科学基金项目(2018A610215);浙江省2021年度高校国内访问工程师“校企合作项目”(FG2021275);浙江省教育厅科研项目(Y202044834)共同资助。

摘　　要：组蛋白乙酰转移酶是组蛋白乙酰化的关键酶,它与组蛋白去乙酰化酶共同作用,调节组蛋白乙酰化的动态过程。研究表明,组蛋白乙酰化在植物的生长发育、抗性胁迫反应过程中有十分重要的调节作用。本研究通过PCR扩增获得了白花真樱组蛋白乙酰转移酶基因hat8。通过生物信息学分析,该基因序列全长1701 bp,编码566个氨基酸,预测分子量为63.62 kD,等电点为8.72,并将其命名为Plhat8。通过对白花真樱不同发育期的转录组测序结果分析发现,该基因在白花真樱愈伤组织发育过程中的表达量下调。在接种致病根癌农杆菌At3后,Plhat8基因在感病品种白花真樱中表达量显著上调,而在抗病品种迎春樱的表达量下降。这些研究结果表明,Plhat8可能参与白花真樱响应根癌农杆菌侵染的过程,为樱花抗根癌病的遗传改良提供理论基础。Histone acetyltransferase is a key enzyme in histone acetylation.It interacts with histone deacetylase to regulate the dynamic process of histone acetylation.Studies have shown that histone acetylation plays an important role in the regulation of plant growth and resistance to stress.In this study,we obtain the histone acetyltransferase gene of Prunus lannesiana cv.Multiplex.Bioinformatics analysis showed that the gene length is 1701 bp.This gene encodes 566 amino acids,and the predicted molecular weight and isoelectric point were 63.62 kD and 8.72,respectively.Then the gene was named Plhat8.The expression of hat8 was significantly down-regulated in the root development of Prunus lannesiana cv.Multiplex.After inoculation of Agrobacterium tumefaciens At3,we observed an upregulation and downregulation of Plhat8 gene expression in susceptible variety of Pr.lannesiana cv.Multiplex and resistance variety of Cerasus discoidea,respectively.Results indicate that Plhat8 might participate in the process of biotic resistance of A.tumefaciens in Pr.lannesiana cv.Multiplex.This study provides a theoretical basis information for genetic improvement of Pr.lannesiana cv.Multiplex to root cancer.

关 键 词：白花真樱 根癌农杆菌 Plhat8 基因克隆 功能分析 

分 类 号：S685.99[农业科学—观赏园艺]