AtCIPK8超表达增强拟南芥耐盐性  

Overexpression of AtCIPK8 Enhances Salt Tolerance of Arabidopsis thaliana

作　　者：朱云峰 刘银弟 谢青 夏金泽 李文佳 王太行 江行玉[1] 周扬[2] Zhu Yunfeng;Liu Yindi;Xie Qing;Xia Jinze;Li Wenjia;Wang Taihang;Jiang Xingyu;Zhou Yang(Hainan Key Laboratory for Biotechnology of Salt Tolerant Crops,School of Tropical Crops,Hainan University,Haikou,570228;Key Laboratory for Quality Regulation of Tropical Horticultural Plants of Hainan Province,School of Horticulture,Hainan University,Haikou 570228)

机构地区：[1]海南大学热带作物学院海南省耐盐作物生物技术重点实验室,海口570228 [2]海南大学园艺学院海南省热带园艺作物品质调控重点实验室,海口570228

出　　处：《分子植物育种》2022年第10期3248-3256,共9页Molecular Plant Breeding

基　　金：海南省基础与应用基础研究计划(自然科学领域)高层次人才项目(2019RC046);国家自然科学基金项目(31660253);国家重点研发计划项目(2018YFD1000500);海南省自然科学基金项目(319MS009,318QN189);海南省教育厅项目(Hnky2021-19,Hys2020-242)共同资助。

摘　　要：AtCIPK8是一种蛋白激酶,通过激活AtSOS1外排Na^(+)活性调节植物对盐胁迫的反应,其突变体对盐胁迫非常敏感。然而,AtCIPK8基因的过表达是否能进一步增强植物的耐盐性还不清楚。本研究通过农杆菌介导的方法将AtCIPK8转入到拟南芥中研究其耐盐性。结果显示,NaCl处理均显著抑制了转基因和野生型(WT)植物种子的萌发,但转基因拟南芥种子在100 mmol/L NaCl处理下的发芽率高于野生型。200 mmol/L NaCl处理下,野生型植株表现出萎蔫、失绿等性状,并且株高、植株鲜重都明显低于转基因植株。与野生型相比,过表达AtCIPK8的转基因植株Na^(+)含量较低,K^(+)含量较高,导致转基因植株在盐胁迫下的K^(+)/Na^(+)比值高于野生型植株。此外,在盐胁迫下,转基因和野生型植株的过氧化程度均有所增加,但在AtCIPK8表达的转基因植株中,NaCl处理诱导的过氧化氢(H_(2)O_(2))和丙二醛(MDA)积累量低于野生型植株。本研究结果表明,AtCIPK8通过调节细胞内离子平衡和活性氧(ROS)稳态来促进转基因植物的生长,提高植物耐盐性。本研究为培育耐盐植物提供一个可能的候选基因。The protein kinase AtCIPK8 regulates the response of plants to salt stress via activating Na^(+)exclusion mediated by AtSOS1.The CIPK8 mutant is sensitive to salinity stress.However,whether the overexpression of AtCIPK8 gene can further enhance plant salt tolerance is still unknown.Herein,AtCIPK8 was transformed into Arabidopsis thaliana to examine its salt tolerance.The germinations of both transgenic and wild-type(WT)plants were inhibited significantly under Na Cl treatment,but the germination rate of transgenic plants was higher than that of WT plants when treated with 100 mmol/L NaCl.The WT plants showed wilting and greenness loss under 200 mmol/L NaCl treatment,and the plant height and fresh weig ht in WT plants were significantly lower than those in transgenic plants.Our results also showed that the plants expressing AtCIPK8 accumulated lower Na^(+) and higher K^(+) contents compared to WT plants,resulting in higher K^(+)/Na^(+) ratio of the transgenic plants under salt stress.Furthermore,the oxidative degree increased in both transgenic and WT plants exposed to salt stress,but the accumulations of hydrogen peroxide(H_(2)O_(2)) and malondialdehyde(MDA)in transgenic plants were lower compared to WT plants.These results indicated that AtCIPK8 promoted the growth of transgenic plants and enhanced salt tolerance by regulating intracellular ion balance and reactive oxygen species(ROS)homeostasis.This study provides a possible candidate gene for the cultivation of salt tolerant plants.

关 键 词：AtCIPK8基因 转基因拟南芥 耐盐性 

分 类 号：Q943[生物学—植物学]