SLAF-seq技术在蔬菜作物中的应用进展  

Progress on Applications of SLAF-seq Technology to Vegetable Crop

作　　者：黄皓[1] 周生茂[1] 吴永升[1] 班美玲 武涛 徐月梅 陈琴 陈振东[1] 黄如葵[1] Huang Hao;Zhou Shengmao;Wu Yongsheng;Ban Meiling;Wu Tao;Xu Yuemei;Chen Qin;Chen Zhendong;Huang Rukui(Vegetable Research Institute of Guangxi Academy of Agricultural Sciences,Nanning,530007;Guangxi Academy of Environmental Protection Sciences,Nanning.530022;Horticultural College of Hunan Agricultural University,Changsha.410128)

机构地区：[1]广西农业科学院蔬菜研究所,南宁530007 [2]广西环境保护科学研究院,南宁530022 [3]湖南农业大学园艺学院,长沙410128

出　　处：《分子植物育种》2022年第10期3306-3312,共7页Molecular Plant Breeding

基　　金：广西科技重大专项(桂科AA17204026);广西重点研发计划项目(桂科AB18216001);广西农业科学院基本科研业务专项项目(桂农科2016YM63,桂农科2021YT106,桂农科2021JM91)共同资助。

摘　　要：特异长度扩增片段测序(specific length amplified fragment sequencing,SLAF-seq)技术是以第二代测序为基础的一种简化基因组测序技术。本研究简述了SLAF-seq技术流程,综述了该技术在蔬菜分子标记开发、高密度遗传图谱构建、基因定位、遗传多样性分析和辅助全基因组测序等方面的应用,讨论了其应用存在的问题,展望了其发展前景。Specific length amplified fragment sequencing(SLAF-seq)technology was one of reduced-representation genome sequencing technology on the basis of the second-generation high-throughput sequencing technology.In this study,the process of SLAF-seq technology was briefly introduced,and the application of SLAF-seq technology in vegetable molecular marker development,high-density genetic map construction,gene mapping,genetic diversity analysis and assisted whole genome sequencing were reviewed.The problems of SLAF-Seq technology were discussed in its applications,and its development prospects were prospected.

关 键 词：SLAF-seq技术 蔬菜作物 分子标记开发 遗传图谱构建 基因定位 

分 类 号：S63[农业科学—蔬菜学]