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作 者:王卓标 方铭 郑乐云[2] 葛辉[2] 陈欣欣 罗辉玉 王志勇[1] WANG Zhuobiao;FANG Ming;ZHENG Leyun;GE Hui;CHEN Xinxin;LUO Huiyu;WANG Zhiyong(Key Laboratory of Healthy Mariculture for the East China Sea,Ministry of Agriculture and Rural Affairs,Fisheries College,Jimei University,Xiamen 361021,China;Key Laboratory of Cultivation and High-value Utilization of Marine Organisms in Fujian Province,Fujian Fisheries Research Institute,Xiamen 361013,China)
机构地区:[1]集美大学水产学院,农业农村部东海海水健康与养殖重点实验室,福建厦门361021 [2]福建省水产研究所,福建省海洋生物增养殖与高值化利用重点实验室,福建厦门361013
出 处:《渔业研究》2022年第3期205-212,共8页Journal of Fisheries Research
基 金:福建省科技计划项目(2019R1013);海水鱼产业技术体系项目(CARS-47-G04).
摘 要:赤点石斑鱼(Epinephelus akaara)是中国东南沿海地区一种重要的海水养殖鱼类,多年来其苗种生产深受神经坏死病的困扰,严重制约了其人工养殖业的发展。本研究采集230尾赤点石斑鱼神经坏死病毒(RGNNV)易感(染病死亡)和230尾抗性(最终健康存活)赤点石斑鱼苗进行基因组重测序,分析获得5412683个单核苷酸多态性(SNPs)位点的基因型,并以之对抗病性状进行了遗传评估和基因组选择研究,获得的估计遗传力均值为0.5662,预测基因组估计育种值(GEBV)均值为0.1543。随机选择不同数量标记对基因组选择准确性影响进行评估,结果表明,采用≥5000个(5 k)标记进行赤点石斑鱼抗神经坏死病性状遗传评估就可以获得比较理想的效果。本研究为开展赤点石斑鱼抗神经坏死病基因组选择育种实践提供了有用的理论参考。Red-spotted grouper(Epinephelus akaara)is an important maricultured fish in the southeast coastal area of China.It is highly infected by red-spotted grouper nerve necrosis virus(RGNNV)during the artificial cultivation,which seriously restricted the development of the artificial breeding industry.In this study,230 ind RGNNV susceptible(disease-infected death)and 230 ind resistant(eventually survived fish)red-spotted grouper fry were collected for genome resequencing,and 5412683 single nucleotide polymorphisms(SNPs)were discovered,which were used for genetic evaluation of the RGNNV resistance of the red-spotted grouper.The estimated heritability of RGNNV was 0.5662,and the accuracy of the genomic estimated breeding value(GEBV)with whole genome SNPs markers was 0.1543.The evaluation of the effect of random selection of different numbers of markers on the genomic prediction accuracy showed that using≥5000(5 k)SNPs markers could obtain more desirable results.This study provided a useful theoretical reference for application of genomic selection for breeding of red-spotted grouper against RGNNV.
关 键 词:赤点石斑鱼 基因组选择 基因组最佳线性无偏预测(GBLUP)
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