细基江蓠繁枝变种原生质体制备条件优化  

Optimization of protoplast isolation conditions of Gracilaria tenuistipitata var.liui Zhang et Xia

作　　者：何艺宾 郝华[2] HE Yibin;HAO Hua(School of Marine Biology,Xiamen Ocean Vocational College,Xiamen 361100,China;State-Province Joint Engineering of Marine Bioproducts and Technology,Xiamen University,Xiamen 361104,China)

机构地区：[1]厦门海洋职业技术学院海洋生物学院,福建厦门361100 [2]厦门大学海洋生物制备技术国家地方联合工程实验室,福建厦门361104

出　　处：《渔业研究》2022年第3期229-234,共6页Journal of Fisheries Research

基　　金：厦门海洋职业技术学院2019年高层次人才科研专项.

摘　　要：细基江蓠繁枝变种(Gracilaria tenuistipitata var.liui Zhang et Xia)俗称细江蓠(Gracilaria crinale),是我国沿海地区主要养殖的大型海洋经济藻类之一。为获得细基江蓠繁枝变种原生质体制备的最佳酶解条件,本研究以酶解液组分、酶解温度和酶解时间等因素对细基江蓠繁枝变种原生质体分离纯化的实验条件进行优化,最终得出最佳酶解条件为:1)酶解液组分:盐度为25的灭菌天然海水中加入10 mmol/L CaCl_(2)、0.8 mol/L D-甘露醇、2%纤维素酶R-10、1%离析酶R-10、0.5%果胶酶Y-23、10 U/mL琼脂糖酶,pH 6.3;2)酶解条件:28℃、80 r/min、黑暗酶解8 h。Gracilaria tenuistipitata var.liui Zhang et Xia,commonly known as Gracilaria crinale,is one of the major marine economic algae cultivated in coastal areas of China.In order to obtain the best enzymolysis conditions for the isolation of protoplasts from Gracilarla tenuistipitata var.liui Zhang et Xia,the isolation conditions of protoplasts were optimized from the factors of enzymolysis solution composition,enzymolysis temperature and enzymolysis time.The optimum enzymatic hydrolysis conditions were as follows:1)Enzymatic hydrolysis solution components:salinity 25 sterilized natural seawater with 10 mmol·L^(-1) CaCl_(2),0.8 mol/L D-Mannitol,2%Cellulase R-10,1%Maecrozyme R-10,0.5%pectinase Y-23,10 U/mL agarase,pH 6.3;2)Enzymolysis conditions:28℃,80 r·min^(-1),dark enzymolysis for 8 h.This study would provide a powerful tool for the genetic improvement of Gracilaria varieties.

关 键 词：细基江蓠繁枝变种 原生质体 分离 优化 

分 类 号：S917.3[农业科学—水产科学]