贮藏方法对紫花苜蓿种子内生根瘤菌传代能力的影响  

Effects of storage methods on the transmissibility of endophytic rhizobia from alfalfa seeds

作　　者：刘畅 师尚礼[1] 康文娟[1] 苗阳阳 吴芳[1] LIU Chang;SHI Shang-li;KANG Wen-juan;MIAO Yang-yang;WU Fang(College of Grassland Science,Gansu Agricultural University,Key Laboratory for Grassland Ecosystem of Ministry of Education,Pratacultural Engineering Laboratory of Gansu Province,Sino-U.S.Centers for Grazing Land Ecosystem Sustainability,Lanzhou 730070,China;College of Animal Science&VeterinaryMedicaine,Heilongjiang Bayi Agricultural University,Daqing 163319,China)

机构地区：[1]甘肃农业大学草业学院,草业生态系统教育部重点实验室,甘肃省草业工程实验室,中-美草地畜牧业可持续发展研究中心,甘肃兰州730070 [2]黑龙江八一农垦大学动物科技学院草业科学系,黑龙江大庆163319

出　　处：《草原与草坪》2022年第2期18-27,共10页Grassland and Turf

基　　金：国家牧草产业技术体系项目(CARS-34)。

摘　　要：为探索贮藏方法对紫花苜蓿种子内生根瘤菌传代能力的影响,将青色荧光蛋白标记根瘤菌gn5f(内源)和12531f(外源)接种于结荚期甘农5号紫花苜蓿。收获的种子,在25℃+自然湿度(S1)、25℃+硅胶干燥(S2)、4℃+冰箱冷藏湿度(S3)和-4℃+冰箱冷冻湿度(S4)条件下贮藏3年,然后播种于大田,次代植株苗期进行内生根瘤菌传代能力和传代有效性检测,以无菌水浇灌苜蓿植株后收获的种子为对照。结果表明:标记根瘤菌gn5f和12531f均可通过种子传代到次代植株。S1贮藏条件下,gn5f在次代植株根、茎、叶的定殖数量显著高于其他处理(P<0.05),次代植株苗期单株结瘤数、单株根瘤重、根瘤等级、固氮酶活性、单株叶片数、株高、根长、地上部分鲜重、地上部分干重、根鲜重、根干重较CK分别增加了37.00%、1183.54%、47.52%、23909.07%、38.72%、40.63%、33.82%、68.11%、137.73%、223.70%、600.00%。S2贮藏条件下,12531f在次代植株根部定殖数量最高,为560.63 cfu/g,次代植株苗期单株结瘤数、根瘤直径、根瘤等级、固氮酶活性、单株叶片数、株高、根长、地上部分鲜重、地上部分干重、根鲜重、根干重较CK分别增加了37.90%、21.33%、27.01%、288.21%、41.22%、39.40%、36.04%、72.37%、144.58%、284.05%、872.04%。适宜的贮藏方法有利于提高种子内生根瘤菌的传代能力和传代有效性,但因根瘤菌株种类和来源而异。gn5f内生种子在S1条件下贮藏、12531f内生种子在S2条件下贮藏分别有助于gn5f和12531f传代到次代植株并高效结瘤固氮,促进次代植株生长;且S2条件贮藏的12531f内生种子形成的次代植株结瘤指标和生长指标显著优于S1条件贮藏的gn5f内生种子形成的次代植株。Seed endophytic rhizobia migration is a new form of sustainable symbiosis system to promote the growth of alfalfa plants.In order to explore the effect of storage methods on the transmissibility of seed endophytic rhizobia,seeds were harvested from Medicago sativa cv.Gannong No.5 plants which were inoculated with green fluorescent protein-labeled rhizobia gn5f(endogenous)and 12531f(exogenous).Before sowing,the seeds were stored for 3 years under the following conditions:25℃+natural humidity(S1),25℃+silica gel(S2),4℃+refrigerator humidity(S3)and-4℃+refrigerator freezing humidity(S4).The control was the seeds harvested from alfalfa plants irrigated with sterile water.The sustainability and effectiveness of endophytic rhizobia were evaluated at the seedling stage of the subsequent generation.Results showed that gn5f and 12531f can be passed from seeds to the next generation plants.Under S1 condition,the number of gn5fcolonized in the roots,stems and leaves of the next generation plants were significantly higher than that under other treatments(P<0.05).At the seedling stage of the next generation plants,compared with the control,the nodule number per plant,nodule weight per plant,nodule grade,nitrogenase activity,leaf number,plant height,root length,above ground fresh weight,above ground dry weight,root fresh weight,root dry weight were increased by 37%,1183.54%,47.52%,23909.07%,38.72%,40.63%,33.82%,68.11%,137.73%,223.70%and 600.00%,respectively.Under S2 condition,12531f had the highest colonization number in the roots of the next generation plants(560.63 cfu/g).The nodule number per plant,nodule diameter,nodule grade,nitrogenase activity,leaf number per plant,plant height,root length,above ground fresh weight,above ground dry weight,root fresh weight,root dry weight were higher than those of control and increased by 37.90%,21.33%,27.01%,288.21%,41.22%,39.40%,36.04%,72.37%,144.58%,284.05%,872.04%,respectively.Appropriate storage methods contributed to the improvement of sustainability and effectiveness of see

关 键 词：紫花苜蓿 种子 贮藏方法 内生根瘤菌 传代能力 

分 类 号：S541[农业科学—作物学]