CASR/VDR/PTH1R信号通路在含钙肾结石发生机制中的初步研究  

A Preliminary Study of CASR/VDR/PTH1R Signaling Pathway in the Pathogenesis of Calcium-containing Kidney Stones

作　　者：李静玲 柯坤彬[1] 王振丞 秦德强 李颢[1] LI Jingling;KE Kunbin;WANG Zhencheng;QIN Deqiang;LI Hao(Dept.of Urinary Surgery,The First Affiliated Hospital of Kunming Medical University,Kunming Yunnan 650032,China)

机构地区：[1]昆明医科大学第一附属医院泌尿外科,云南昆明650032

出　　处：《昆明医科大学学报》2022年第6期13-18,共6页Journal of Kunming Medical University

基　　金：云南省高层次卫生技术人才项目(D-2017043);云南省科技厅科技计划项目(202001AY070001-158);云南省科技厅基金委员会项目(2019FE001-064)。

摘　　要：目的研究CASR/VDR/PTH1R通路在含钙肾结石发生中的作用及机制。方法取肾结石患者肾脏髓质为实验组,肾脏肿瘤患者正常肾髓质为对照组,分别采用q-PCR、WB、免疫荧光法检测肾实体组织中CASR、VDR、PTH1R的mRNA表达和蛋白水平。结果CASR、PTH1R基因mRNA在积水肾髓质及肾肿瘤正常髓质表达水平差异无统计学意义(P>0.05)。VDR基因的mRNA在积水肾髓质及肾肿瘤正常髓质表达水平差异有统计学意义(P<0.05)。结论CASR、VDR、PTH1R在肾结石患者肾脏髓质中表达,且有可能通过CASR/VDR/PTH1R信号通路途径参与含钙肾结石的发生机制。Objective To study the role and mechanism of CASR,VDR,PTH1R and other genes in the occurrence of calcium-containing kidney stones.Methods The renal medulla of patients with renal calculi was selected as the experimental group,and the normal renal medulla of patients with renal tumors was selected as the control group.The mRNA expression and protein level of CASR,VDR and PTH1R in renal solid tissue were detected by q-PCR,WB and immunofluorescence,respectively.Results There was no significant difference in the expression levels of CASR and PTH1R gene mRNA in the hydronephrotic medulla and the normal medulla of renal tumor(P>0.05).The difference of VDR gene mRNA expression in hydronephrotic medulla and normal medulla of renal tumor was statistically significant(P<0.05).Conclusion CASR,VDR,and PTH1R are involved in the occurrence of calcium-containing kidney stones,and may participate in the mechanism of calciumcontaining kidney stones through the CASR/VDR/PTH1R signaling pathway.

关 键 词：钙敏受体 维生素D受体 重组人甲状旁腺激素1受体 基因表达 肾结石 

分 类 号：R692.4[医药卫生—泌尿科学]