促渗剂对八桂止痛乳膏体外经皮吸收的影响  

Effects of Different Penetration Enhancers on in vitro Transdermal Permeation of Bagui Zhitong Cream

作　　者：张锦丽 梅佳华 廖承成 胡丹 黄虹 李元文[4] ZHANG Jinli;MEI Jiahua;LIAO Chengcheng;HU Dan;HUANG Hong;LI Yuanwen(Dept.of Dermatology,Lijiang County Hospital of Traditional Chinese Medicine,Lijiang Yunnan 674100;College of Traditional Chinese Medicine,Yunnan Univerisity of Chinese Medicine,Kunming Yunnan 650500;First School of Clinical Medicine of Yunnan Univerisity of Chinese Medicine,Kunming Yunnan 650021,China;Dept.of Dermatology,Dongfang Hospital,Beijing University of Chinese Medicine,Beijing 10000,China)

机构地区：[1]丽江市中医医院皮肤科,云南丽江674100 [2]云南中医药大学中药学院,云南昆明650500 [3]云南中医药大学第一临床医学院,云南昆明650021 [4]北京中医药大学东方医院皮肤性病科,北京100000

出　　处：《昆明医科大学学报》2022年第6期19-24,共6页Journal of Kunming Medical University

基　　金：云南省科技厅中医联合项目-面上项目(202001AZ070001-053);云南省中医医院卓越人才项目(云中附党字[2021]5号)。

摘　　要：目的研究八桂止痛乳膏的最佳制备方法,确定最佳基质配方,考察不同浓度促渗剂氮酮对八桂止痛乳膏体外透皮吸收的影响。方法制备含不同浓度促渗剂的八桂止痛乳膏。采用Franz扩散池法,离体小鼠皮肤为经皮吸收材料,高效液相色谱法作为此研究测定方法,比较桂皮醛在不同浓度促渗剂下的累积渗透量(Q)、经皮吸收速率(J),以此为指标,筛选出最佳促渗剂浓度。结果不同浓度的氮酮均不同程度地促进桂皮醛体外渗透,其促渗顺序为:5%氮酮组>3%氮酮组>7%氮酮组>1%氮酮组>10%氮酮组。经多重比较,差异均有统计学意义(P<0.05)。结论在含1%、3%、5%、7%和10%氮酮的八桂止痛乳膏中,选择5%的氮酮作为八桂止痛乳膏的促渗剂最佳。Objective To study the best preparation method of Bagui Zhitong cream,to determine the best matrix formulation,and to investigate the effect of different Penetration Enhancers on in vitro Transdermal Permeation of Bagui Zhitong Cream.Methods We prepared Bagui Zhitong Cream with different concentrations of penetration enhancer of azone.Modified Franz diffusion cell was adopted using mouse skin of isolated male rat as transdermal barrier.The content of Cinnamaldehyde was determined by UPLC.The accumulative transdermal amount(Q)and percutaneous permeability(J)were calculated and compared between different concentrations of BaGui Zhitong cream to select the best concentration of Penetration Enhancers.Results Different concentrations of azone promoted Cinnamaldehyde in vitro permeation in different degrees,the order of promoting infiltration was as follows:5%azone>3%azone>7%azone>1%azone>10%azone.Through the multiple comparison,the difference had statistical significance(P<0.05).Conclusion In Bagui Zhitong cream containing 1%azone,3%azone,5%azone,7%azone and 10%azone,5%azone as penetration enhancer has the best effect in Bagui Zhitong Cream.

关 键 词：八桂止痛乳膏 促渗剂 体外经皮吸收 桂皮醛 氮酮 

分 类 号：R283.6[医药卫生—中药学] R284.1[医药卫生—中医学]