冠心病急性心肌梗死患者外周血差异基因表达分析及功能  

Analysis of Differential Gene Expression and Function in Peripheral Blood of Patients with Coronary Heart Disease and Acute Myocardial Infarction

作　　者：苗文清[1,2] 王宇[1] 赵晓丽[3] 田倪妮 尤丽英[2] MIAO Wenqing;WANG Yu;ZHAO Xiaoli;TIAN Nini;YOU Liying(Dept.of Cardiovascular Medicine,Kunming First People’s Hospital,Kunming Yunnan 650011,China;Dept.of General Medicine,Kunming First People’s Hospital,Kunming Yunnan 650011,China;Clinical Laboratory,Kunming First People’s Hospital,Kunming Yunnan 650011,China)

机构地区：[1]昆明市第一人民医院心血管内科,云南昆明650011 [2]昆明市第一人民医院全科医学科,云南昆明650011 [3]昆明市第一人民医院检验科,云南昆明650011

出　　处：《昆明医科大学学报》2022年第6期25-34,共10页Journal of Kunming Medical University

基　　金：昆明市卫生健康委员会卫生科研课题(2020-03-01-111);云南省教育厅科学研究基金资助项目(2022J0267)。

摘　　要：目的探讨冠心病急性心肌梗死患者外周循环血单核细胞中差异基因表达及功能。方法收集2020年9月至2021年9月在昆明市第一人民医院接受冠脉造影的患者,提取外周血单核细胞总RNA,使用DNBSEQ平台进行第2代高通量测序。检测并筛选差异表达基因,进行KEGG及GO富集分析。结果冠心病急性心肌梗死组与冠脉正常组对比,其中差异基因89个,上调基因67个,下调基因22个;其中差异lncRNA14个,差异mRNA72个,差异circRNA3个。完成KEGG pathway富集分析、KEGG disease富集分析、KEGG molecular富集分析。完成GO cell composition富集分析、GO cell function富集分析、GO biological process molecular富集分析。结论筛选出显著差异的10个mRNA、6个lncRNA、2个circRNA。富集分析中涉及感染、转录失调、PI3K-Akt信号通路、脂类合成、吞噬泡腔、细胞外基质、脂多糖结合等,与冠状动脉粥样硬化的发生与发展及急性血栓事件的发生可能相关。Objective To investigate the differential gene expression and function of peripheral blood monocytes in patients with coronary heart disease and acute myocardial infarction.Methods Patients receiving coronary angiography in Kunming First People’s Hospital from September 2020 to September 2021 were collected and total RNA of peripheral blood monocytes was extracted.DNBSEQ platform was used for second-generation highthroughput sequencing.We tested and screened differentially expressed genes,and made KEGG and GO enrichment analysis.Results There were 89 differentially expressed genes,67 up-regulated genes and 22 downregulated genes in acute myocardial infarction group compared with normal coronary artery group.Among them,there were 14 differential lncRNAs,72 differential mRNAs,and 3 differential circRNAs.KEGG pathway enrichment analysis,KEGG disease enrichment analysis and KEGG molecular enrichment analysis were completed.GO cell composition enrichment analysis,GO cell function enrichment analysis and GO biological process molecular enrichment analysis were completed.Conclusions 10 mRNAs,6 lncRNAs,and 2 circRNAs with significant differences were screened.The enrichment analysis involves infection,transcriptional disorder,PI3K Akt signaling pathway,lipid synthesis,phagocytic vesicle cavity,extracellular matrix,lipopolysaccharide binding,etc.,which may be related to the occurrence and development of coronary atherosclerosis and the occurrence of acute thrombotic events.

关 键 词：冠心病 急性心肌梗死 差异基因表达分析 mRNA、lncRNA、circRNA KEGG富集分析 GO富集分析 

分 类 号：R541[医药卫生—心血管疾病]