白细胞介素-33对巨噬细胞羧酸酯酶作用的体外研究  

In Vitro Effects of Interleukin-33 on Carboxylesterases in Macrophages

作　　者：李敏[1] 徐艳娇 张程亮 祖越 高前艳 王熙敏 陈云舟 LI Min;XU Yanjiao;ZHANG Chengliang;ZU Yue;GAO Qianyan;WANG Ximin;CHEN Yunzhou(Department of Pharmacy,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China)

机构地区：[1]华中科技大学同济医学院附属同济医院药学部,武汉430030

出　　处：《医药导报》2022年第6期768-773,共6页Herald of Medicine

基　　金：国家自然科学基金资助项目(81670521,81803798)。

摘　　要：目的 研究白细胞介素(IL)-33对RAW264.7巨噬细胞羧酸酯酶(CESs)的作用和细胞极化状态的影响。方法 以不同浓度IL-33刺激RAW264.7细胞,通过Western blotting检测CESs的蛋白表达,CCK-8检测CESs活性变化,油红O检测细胞脂质沉积。检测M1型巨噬细胞标志物诱导型一氧化氮合酶iNOS、IL-1β、肿瘤坏死因子-α(TNF-α)和M2型巨噬细胞标志物CD206、IL-10等的表达,评价其极化状态。结果 IL-33剂量依赖性地显著下调巨噬细胞CES1的蛋白表达水平,且对CES1和CES2的代谢活性均产生抑制作用(P<0.05);IL-33干预后,巨噬细胞中三酰甘油(TG)、总胆固醇(TC)及低密度脂蛋白胆固醇(LDL-C)等出现不同程度升高(P<0.05)。IL-33刺激后,RAW264.7小鼠巨噬细胞的CD206蛋白表达显著增多,而iNOS的蛋白表达无明显变化。结论 IL-33刺激后促进巨噬细胞向M2型转化,显著抑制RAW264.7巨噬细胞中CES1的表达及活性,增加巨噬细胞脂质沉积。Objective To investigate the effects of interleukin-33(IL-33)on carboxylesterases(CESs)and cell polarization state in RAW264.7 macrophages.Methods RAW264.7 cells were stimulated with different concentrations of IL-33,and the protein expression of CESs was detected by Western blotting.CCK8 assay was used to detect the activity of CESs and lipid deposition was detected by oil red O.The mRNA expressions of M1 macrophage markers induced nitric oxide synthase(iNOS),IL-1β,TNF-α,and M2 macrophage markers CD206 and IL-10 were detected to identify the polarization of macrophages.Results IL-33 significantly down-regulated the protein expression level of macrophages CES1 in a dose-dependent manner,and inhibited the metabolic activities of CES1 and CES2(P<0.05).After the IL-33 intervention,triglyceride,total cholesterol and low-density lipoprotein in macrophages had increased(P<0.05).Moreover,the expression of CD206 in RAW264.7 mouse macrophages was significantly increased,while the expression of iNOS was not changed.Conclusion IL-33 exerted an M2-polarization effect on RAW264.7 macrophages,and significantly inhibited the expression and activity of CES1 in RAW264.7 macrophages,causing lipid deposition in macrophages.

关 键 词：白细胞介素-33 巨噬细胞 羧酸酯酶 脂质代谢 

分 类 号：R966[医药卫生—药理学]