镧系Eu3+配合物修饰的分子印迹聚合物荧光探针制备及其对血红蛋白的传感检测  

Preparation of Molecularly Imprinted Polymer Fluorescence Probe Modified by Lanthanide Eu^(3+) Complex and Hemoglobin Sensing Detection

作　　者：刘丽[1] 胡润泽 徐陈 徐丛泽 李颖[1] LIU Li;HU Run-ze;XU Chen;XU Cong-ze;LI Ying(School of Materials&Chemistry,University of Shanghai for Science and Technology,Shanghai 200093,China)

机构地区：[1]上海理工大学材料与化学学院,上海200093

出　　处：《发光学报》2022年第6期944-951,共8页Chinese Journal of Luminescence

基　　金：国家自然科学基金(21101107,51173107);污染控制与资源化国家重点实验室基金项目(PCRRF19017)资助。

摘　　要：采用溶胶凝胶法将分子印迹聚合物(MIPs)与镧系Eu^(3+)配合物功能化的离子液体([Eu(BFA)_(3)]@DPA⁃PA)进行化学键合制备得到了基于镧系配合物修饰的分子印迹聚合物探针(Eu⁃MIPs)。利用FT⁃IR、XPS、UV⁃Vis、PL等表征方法对探针分子的结构和性能进行分析。研究结果表明,Eu⁃MIPs荧光聚合物具有优异的荧光性能。此外,通过进一步研究Eu⁃MIPs对血红蛋白(Hb)的荧光传感性能发现,Hb能够对Eu⁃MIPs的荧光产生明显的猝灭效果,这可能是由于Hb和离子液配体对紫外光具有竞争性吸收,进而使配体⁃稀土之间的能量传递过程受到影响,从而造成Eu⁃MIPs的荧光猝灭。同时,Eu⁃MIPs对Hb具有较强的选择性和抗干扰能力,有望用作特异性检测Hb的荧光探针。Molecularly imprinted polymer probes(Eu-MIPs)modified by lanthanide complexes were prepared by chemical bonding of molecularly imprinted polymers(MIPs)and lanthanide Eu^(3+)complex-functionalized ionic liquid([Eu(BFA)_(3)]@DPA-PA)by sol-gel method.The structure and properties of the probe molecules were analyzed by characterization methods such as FT-IR,XPS,UV-Vis,and PL.The results show that the Eu-MIPs fluorescent polymer has excellent fluorescence properties.In addition,by further studying the fluorescence sensing performance of Eu-MIPs for hemoglobin(Hb),it was found that Hb can produce a significant quenching effect on the fluorescence of Eu-MIPs,which may be due to the competitive absorption of UV light by Hb and ionic liquid ligands,and then the energy transfer process between ligands and rare earths is affected,resulting in the fluorescence quenching of Eu-MIPs.At the same time,Eu-MIPs have strong selectivity and anti-interference ability to Hb,and are expected to be used as fluorescent probes for the specific detection of Hb.

关 键 词：分子印迹 镧系配合物 荧光探针 传感检测 

分 类 号：TB333[一般工业技术—材料科学与工程]