超高效液相色谱-蒸发光散射法测定石油采出液中十八烷基羟基磺基甜菜碱  

Determination of octadecyl hydroxy sulfobetaine in petroleum extraction liquid by UPLC-evaporative light scattering detector

作　　者：李润植 田茂章[2] 张虹[3] 杨凤敏[3] 沈刚[3] 汪长征 唐亚林[3] LI Runzhi;TIAN Maozhang;ZHANG Hong;YANG Fengmin;SHEN Gang;WANG Changzheng;TANG Yalin(School of Environment and Energy Engineering,Beijing University of Civil Engineering and Architecture,Beijing 100044,China;State Key Laboratory of Enhanced Oil Recovery,Research Institute of Petroleum Exploration&Development,Beijing 100083,China;Institute of Chemistry,Chinese Academy of Sciences,Beijing 100190,China)

机构地区：[1]北京建筑大学环境与能源工程学院,北京100044 [2]中国石油勘探开发研究院提高石油采收率国家重点实验室,北京100083 [3]中国科学院化学研究所,北京100190

出　　处：《分析试验室》2022年第5期583-587,共5页Chinese Journal of Analysis Laboratory

基　　金：中石油科学技术院项目(RIPED.CN-2020-JS-257)资助。

摘　　要：采用超高效液相色谱-蒸发光散射检测(UPLC-ELSD)技术,建立了测定石油采出液中十八烷基羟基磺基甜菜碱(OHSB)含量的方法。选用C_(18)色谱柱,以水-甲醇为流动相,流速为0.3 mL/min,柱温为40℃,梯度洗脱,ELS为检测器,漂移管温度70℃,检测器增益为50,喷雾器载气压力241.3 kPa。研究表明,OHSB质量浓度在5~50 mg/L范围内,峰面积与浓度符合二次方程A=96.888c^(2)+133.1c-29.684,相关系数R2为0.997,检出限为1.2 mg/L。油田地层水加样回收实验的回收率分别为95.0%,100.6%,101.0%,相对标准偏差(RSD)均在3%以内。影响因素实验表明,石油磺酸盐(KPS)、聚丙烯酰胺、高浓度无机盐对检测方法几乎无影响,可满足实际应用需求。该方法为石油采出液样品分析检测提供有效手段。A method was developed for the quantitative detection of octadecyl hydroxy sulfobetaine(OHSB)in petroleum extraction liquid by ultra performance chromatography-evaporative light scattering detector(UPLCELSD).The chromatographic separation was conducted on a C_(18) column using deionized water and methanol as mobile phase for gradient elution.The flow rate was 0.3 mL/min and column temperature was 40℃.The drift tube temperature of the ELS was set at 70℃and gas pressure of a sprayer carrier was set at 241.3 kPa.It was found that when the concentration of OHSB was in the range of 5-50 mg/L,the relationship of the concentration and peak area conformed to the quadratic equation:A=96.888c2+133.1c-29.684,whose R2 value was 0.997.The detection limit was 1.2 mg/L.The recoveries of the oilfield formation water samples were 95.0%,100.6%and 101.0%,respectively,and all the ralative standard deviations(RSDs)were within 3%.The experimental results of the influencing factors showed that petroleum sulfonate(KPS),polypropylene amide and high-concentration inorganic salts had almost no effect on the detection method,which can meet the needs of practical applications.The method provides an effective means for the analysis and detection of petroleum produced fluid samples.

关 键 词：超高效液相色谱 蒸发光散射检测器 羟基磺基甜菜碱 定量分析 

分 类 号：X832[环境科学与工程—环境工程] O657.7[理学—分析化学]