某猪场非洲猪瘟临床病理变化及病毒组织分布观察  

Clinical pathological characteristics of African swine fever and tissue distribution of the virus in a swine farm

作　　者：夏婷婷 高雁怩 白娟[1] 王俊荣 张路捷 姜平[1] XIA Tingting;GAO Yanni;BAI Juan;WANG Junrong;ZHANG Lujie;JIANG Ping(Nanjing Agricultural University/Key Laboratory of Animal Bacteriology,Ministry of Agriculture and Rural Affairs,Nanjing 210095,China)

机构地区：[1]南京农业大学/农业农村部动物细菌学重点实验室,江苏南京210095

出　　处：《畜牧与兽医》2022年第6期104-107,共4页Animal Husbandry & Veterinary Medicine

基　　金：国家生猪产业技术体系专项(CARS-35);国家自然科学基金(32002266);十四五国家重点研发计划子课题(2021YFD1801305-3)。

摘　　要：选择某疑似非洲猪瘟(African swine fever,ASF)临床发病猪场3头病死仔猪,系统观察其眼观病变、组织病理学变化,检测非洲猪瘟病毒(ASFV)及其在不同组织中的分布。结果显示:发病死亡仔猪内脏器官广泛出血与肿大,其中以淋巴结与脾脏病变最为显著。内脏器官组织学病理变化以广泛性的充血、出血及炎性细胞浸润为主。实时荧光定量PCR与免疫组化显示脾脏、淋巴结、肝脏和肺脏中病毒载量较大,心脏、肾脏和脑次之。组织中的病毒载量与组织病变程度具有一定相关性,证明ASFV感染猪体全身组织,可造成严重的血液循环障碍与炎症反应,为该病发病机理研究提供了理论依据。Three diseased and dead piglets were collected from a swine farm with doubtable clinical signs for African swine fever in this study.The autopsy changes and histopathological characteristics of the dead piglets were observed,and the distribution of African swine fever virus(ASFV)in them was detected in the different organ tissues.Macroscopic lesion showed extensive hemorrhage and swelling in their organs,especially in their lymph nodes and spleen.The histopathological changes were characterized as congestion,bleeding and inflammatory cell infiltration.qPCR and immunohistochemical experiments demonstrated that ASFV existed in the spleen,lymph nodes,liver,and lung of the animals at higher levels,as compared with those in their heart,kidney and brain.These phenomena indicated that ASFV infected all the organs of the diseased piglets and caused severe systemic blood circulation disorder and inflammatory reaction.The study provided useful data for exploration of the pathogenesis of ASFV.

关 键 词：非洲猪瘟 病理变化 病毒分布 

分 类 号：S855.3[农业科学—临床兽医学]