高效液相色谱法测定水体中的痕量一氧化氮  

High performance liquid chromatography method for the determination of trace nitric oxide in water

作　　者：范岩松 刘春颖[1] 田野[1,2] 李培峰 贡江琛[1] 史雅琦 FAN Yansong;LIU Chunying;TIAN Ye;LI Peifeng;GONG Jiangchen;SHI Yaqi(Key Laboratory of Marine Chemistry and Theory,Ministry of Education,College of Chemistry and Chemical Engineering,Ocean University of China,Qingdao 266100,China;School of Marine Science,Nanjing University of Information Science&Technology,Nanjing 210044,China)

机构地区：[1]中国海洋大学海洋化学与理论教育部重点实验室,化学化工学院,青岛266100 [2]南京信息工程大学海洋科学学院,南京210044

出　　处：《分析试验室》2022年第6期655-659,共5页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金项目(41676065);国家重点研究发展计划项目(2016YFA0601301)资助。

摘　　要：基于4,5-二氨基荧光素与一氧化氮(NO)反应生成高荧光三唑荧光素的原理,建立了一种测定水体中溶解NO的高效液相色谱方法。通过不同流动相(乙腈∶磷酸盐缓冲溶液)配比实验和色谱柱温度梯度实验,确定了该方法测定水体中NO的最佳实验条件:流动相配比为乙腈∶磷酸盐缓冲溶液=8∶92(V/V);柱温为25℃。在优化条件下,测得NO浓度与荧光强度在0.33~3.32 nmol/L和13.3~266 nmol/L范围内具有良好的线性关系,检出限为90 pmol/L,相对标准偏差为5.1%,平均加标回收率为122.4%。方法可用于测定可口可乐、植物饮料、海水和日本星杆藻藻液中的NO浓度。Based on the principle that the reaction between 4,5-diaminofluorescein and NO could produce high fluorescent triazolofluorescein,a high performance liquid chromatography method for the determination of dissolved NO in water was proposed.The effects of different mobile phase(acetonitrile:phosphate buffer solution)ratio and chromatographic column temperature gradient were investigated.The experimental results indicated that the optimum experimental conditions for the determination of NO in water were as follows:the mobile phase of acetonitrile∶phosphate buffer of 8∶92(V/V),and the column temperature of 25℃.There were good linear relationships between the NO concentration and the fluorescence intensity in the ranges of 0.33-3.32 and 13.3-266 nmol/L.The detection limit was 90 pmol/L,the relative standard deviation was 5.1%,and the average standard recovery was122.4%.The method worked well in determining the concentration of NO in the Coca Cola,plant drink,Qingdao Shilaoren seawater and the culture medium of Stellaria japonicum.

关 键 词：一氧化氮 高效液相色谱法 4 5-二氨基荧光素 

分 类 号：O657.7[理学—分析化学]