多维功能作图在胡杨幼苗生长QTL的应用  

Application of multi-trait functional mapping of QTL in Populus euphratica Oliv.seedlings

作　　者：穆帅成 祝绪礼 叶梅霞 邬荣领 Mu Shuai-Cheng;Zhu Xu-Li;Ye Mei-Xia;Wu Rong-Ling(Center for Computational Biology, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China;Center for Statistic Genetics Departments of Public Health Sciences and Statistics, Pennsylvania State University, Hershey, PA 17033, USA)

机构地区：[1]北京林业大学生物科学与技术学院计算生物学中心,北京100091 [2]宾夕法尼亚州立大学,医学院公共健康科学系和统计系,统计遗传中心,宾夕法尼亚州17033

出　　处：《植物科学学报》2022年第3期365-377,共13页Plant Science Journal

基　　金：国家自然科学基金(32071796);中国博士后科学基金(2019M660496);北京林业大学科技创新计划(BLX201912)。

摘　　要：功能作图框架能够表征复杂动态性状背后的数量性状位点(Quantitative trait locus,QTL)或核苷酸。此前,功能作图广泛应用于单个性状或两个性状的QTL定位当中,多个相关性状功能作图的研究相对匮乏。本研究通过对SAD(Structured antedependence)模型进一步推导,得到在多个性状时用于拟合时间相关协方差矩阵的结构化模型,并使用胡杨(Populus euphratica Oliv.)340个F_(1)代群体的4个生长相关性状以及模拟实验对多维功能作图模型进行测试。结果显示,共定位出173个显著位点,分布于除4、9、10、15、16以外的染色体。功能注释涉及参与叶绿体早期发育、生长素横向运输、提高植物非生物胁迫耐受性以及作为跨膜运输系统开关等生物学过程的32个基因。Functional mapping frameworks can characterize quantitative trait loci(QTL)or nucleotides underlying complex dynamic traits.Functional mapping has been widely used in QTL mapping of a single trait or two traits,but research on multiple correlated traits remains relatively scarce.In this study,the structured antedependence(SAD)model was further derived and used to fit time-dependent covariance matrices for multiple traits.Four growth related traits of 340 Populus euphratica Oliv.F_(1) populations as well as simulation experiments were used to test this multi-trait functional mapping model.In total,173 significant loci were located on the chromosomes,except for 4,9,10,15,and 16.Functional annotation identified 32 genes involved in early chloroplast development,lateral auxin transport,abiotic stress tolerance,and transmembrane transport system switching.

关 键 词：多维功能作图 数量性状位点 结构前依赖模型 胡杨 关联分析 

分 类 号：Q-3[生物学]