三叶木通转录组分析及三萜皂苷生物合成途径关键酶基因的发掘  

Analysis of the transcriptome and discovery of key enzyme genes of the triterpenoid saponin biosynthesis pathway in Akebia trifoliata(Thunb.)Koidz.

作　　者：钱程程 赵历强 杨亚湉 韩邦兴[3] 吴家文 欧金梅 Qian Cheng-Cheng;Zhao Li-Qiang;Yang Ya-Tian;Han Bang-Xing;Wu Jia-Wen;Ou Jin-Mei(School of Pharmacy, Anhui University of Chinese Medicine, Hefei 230012, China;Key Laboratory of Xin'an Medicine, Ministry of Education, Experimental Center for Scientific Research, Anhui University of Chinese Medicine, Hefei 230038, China;College of Biological and Pharmaceutical Engineering, West Anhui University, Lu'an, Anhui 237012, China;Key Laboratory of New Technology of Chinese Herbs Pieces Manufacturing, Hefei 230012, China)

机构地区：[1]安徽中医药大学药学院,合肥230012 [2]安徽中医药大学科研实验中心,新安医学教育部重点实验室,合肥230038 [3]皖西学院生物与制药工程学院,安徽六安237012 [4]中药饮片制造新技术安徽省重点实验室,合肥230012

出　　处：《植物科学学报》2022年第3期378-389,共12页Plant Science Journal

基　　金：安徽中医药大学人才项目(2021rcyb011);中央财政林业科技推广示范资金项目(Z175070050002);安徽高校自然科学研究项目(KJ2019A0479)。

摘　　要：为了解三叶木通(Akebia trifoliata(Thunb.)Koidz.)的三萜皂苷合成途径及其关键酶,本研究对其花、叶、根、茎进行转录组测序,组装获得了57.25 Gb数据,含140859个unigenes,序列平均长度为1350 bp。KEGG代谢通路富集结果显示,517个unigenes参与三萜皂苷合成相关的3条代谢途径,其中415个unigenes编码三萜皂苷生物合成途径的19个关键酶。对三萜皂苷生物合成过程中的关键酶角鲨烯环氧酶(SE)进行序列分析和同源建模,发现其具有保守的底物结合结构域。将三叶木通茎与花、叶、根的基因表达水平进行比较,发现茎与根相比较其上调基因数目最多,其中295个差异表达基因(DEGs)与三萜皂苷生物合成途径相关。To understand the biosynthesis pathway and key enzymes of triterpenoid saponins in Akebia trifoliata(Thunb.)Koidz.,transcriptome sequencing was performed on flowers,leaves,roots,and stems using the BGI Illumina HiSeq sequencing platform.After assembly,we obtained 57.25 Gb of data,including 140859 unigenes with an average sequence length of 1350 bp.KEGG pathway enrichment analysis identified 517 unigenes involved in three metabolic pathways related to triterpenoid saponin biosynthesis,of which 415 unigenes encoded 19 key enzymes of the triterpenoid saponin biosynthesis pathway.Sequence analysis and homology modeling of squalene epoxide(SE),a key enzyme of triterpenoid saponin biosynthesis,revealed a conserved substrate-binding domain.Comparing gene expression levels in the stem,flower,leaf,and root of Akebia trifoliata showed that up-regulated genes were the richest between the stem and root,including 295 differentially expressed genes(DEGs)related to the triterpenoid saponin biosynthesis pathway.

关 键 词：三叶木通 转录组测序 三萜皂苷 生物合成 角鲨烯环氧酶 

分 类 号：Q943.2[生物学—植物学]