小动物活体光学三维成像系统及其对乳腺癌的定量分析  

Small Animal Living Three-dimensional Optical Imaging System and Its Quantitative Analysis of Breast Cancer

作　　者：郑南南 黄钢[2] ZHENG Nannan;HUANG Gang(School of Health Science and Engineering,University of Shanghai for Science and Technology,Shanghai 200093,China;Shanghai Key Laboratory of Molecular Imaging,Shanghai University of Medicine and Health Sciences,Shanghai 201318,China)

机构地区：[1]上海理工大学健康科学与工程学院,上海200093 [2]上海健康医学院,上海市分子影像学重点实验室,上海201318

出　　处：《激光生物学报》2022年第3期215-223,共9页Acta Laser Biology Sinica

基　　金：国家自然科学基金项目(82127807);上海市分子影像学重点实验室建设项目(18DZ2260400)。

摘　　要：乳腺癌具有高转移率。使用小动物活体成像技术对乳腺癌的生长及转移情况实时监测定量分析可以帮助了解疾病机制及进行药物研究。二维成像对光学信号的定位与定量是相对的。随着计算机技术的进步,可以实现对采集的图像进行三维重建,精准量化光学信号,获得空间分布的三维信息。IVIS Spectrum小动物活体光学三维成像系统同时具有高灵敏的生物发光、荧光、切伦科夫辐射二维成像及三维扫描重建功能,是小动物活体光学成像的顶级系统。本文对人乳腺癌细胞(MDA-MB-231)进行慢病毒感染,在体外稳定表达荧光素酶后,选取重度联合免疫缺陷(SCID)小鼠进行原位乳腺癌模型的建立,通过IVIS Spectrum小动物活体光学三维成像系统对小鼠进行生物发光二维成像,无创观测肿瘤的生长及转移情况。本文的创新点是利用生物发光成像断层扫描技术对小鼠模型进行定量三维成像,使用系统自带的算法直接进行三维重建,同时结合鲸鱼优化算法(WOA)优化后的三维卷积的深度编码器-解码器的网络模型进行重建。通过CT图像验证两者的重建效果,得到肿瘤的深度信息,实现对乳腺癌的精准定量分析。Breast cancer has a high metastasis rate. Quantitative analysis of breast cancer growth and metastasis by real-time imaging using small animal imaging technology can help understand the disease mechanism and help drug research. Two dimensional imaging can not achieve high precision positioning and quantification of optical signals. With the progress of computer technology, it can realize the three-dimensional reconstruction of the collected images, accurately quantify the optical signals and obtain the three-dimensional information of spatial distribution. IVIS spectrum small animal in vivo optical three-dimensional imaging system has high sensitive bioluminescence, fluorescence, cherenkov radiation two-dimensional imaging and three-dimensional scanning reconstruction functions. It is the top system of small animal in vivo optical imaging. In this study, human breast cancer cells(MDA-MB-231) were infected with lentivirus. After stable expression of luciferase, the animal models of severely combined immunodeficiency(SCID) were established. The bioluminescence two-dimensional imaging of the mice was carried out through the IVIS Spectrum living animal optical three-dimensional imaging system. The growth and metastasis of the tumor were observed noninvasively. The innovation of this paper is to make quantitative three-dimensional imaging of breast cancer mice by bioluminescence tomography. The algorithm is directly used for 3D reconstruction with the algorithm of the system. The network model of the deep encoding decoder combined with the optimized convolution of WOA is reconstructed. The reconstruction effect is verified by CT diagram, and the depth information of the tumor is obtained, and the accurate quantitative analysis of breast cancer is achieved.

关 键 词：乳腺癌 荧光素酶 生物发光成像 信号源重建 

分 类 号：Q631[生物学—生物物理学]