生脉注射液对心肌缺血再灌注损伤大鼠线粒体融合素2的影响及保护作用  

Effect and Protection of Shengmai Injection on Mitochondrial Fusion-2 in Rats with Myocardial Ischemia Reperfusion Injury

作　　者：张婷 马喆 赵久丽 金秋硕[1] 娄利霞[1] ZHANG Ting;MA Zhe;ZHAO Jiuli;JIN Qiushuo;LOU Lixia(Laboratory of Chinese Internal Medicine of Ministry of Education and Beijing,Dongzhimen Hospital,Beijing University of Chinese Medicine,Beijing 100700,China)

机构地区：[1]北京中医药大学东直门医院中医内科学教育部和北京市重点实验室,北京100700

出　　处：《中西医结合心脑血管病杂志》2022年第11期1959-1964,共6页Chinese Journal of Integrative Medicine on Cardio-Cerebrovascular Disease

基　　金：北京中医药大学在读研究生项目(No.2019-JYB-XS-155);北京中医药大学新奥奖励基金课题(No.2017-XA-JLJJ-012)。

摘　　要：目的探讨生脉注射液对于心肌缺血再灌注损伤大鼠线粒体融合素2(Mfn2)的影响及保护作用机制。方法选取无特定病原体(SPF)级雄性SD大鼠18只,随机分为假手术组、模型组和生脉注射液组,每组6只。采用结扎左冠状动脉前降支30 min再灌注2 h的方法制备急性心肌缺血再灌注损伤大鼠模型。假手术组和模型组大鼠连续7 d每天腹腔注射生理盐水12 mL/kg,生脉注射液组大鼠术前连续7 d每天腹腔注射生脉注射液12 mL/kg。小动物超声检测左室射血分数(LVEF)、左室短轴缩短率(LVFS)、左室后壁舒张末厚度(LVPWTd)、左室后壁收缩末厚度(LVPWTs)、左室舒张末期容量(LVEDV)、左室收缩末期容量(LVESV),末端脱氧核苷酸转移酶介导的缺口末端标记(TUNEL)法检测大鼠心肌细胞凋亡情况,荧光探针法检测大鼠心肌组织超氧化物歧化酶(SOD)活力和丙二醛(MDA)含量,逆转录聚合酶链式反应(RT-PCR)检测大鼠心肌组织Mfn2 mRNA的表达,蛋白免疫印迹法(Western Blot)检测Mfn2蛋白、Caspase-3蛋白的表达。结果模型组LVEF、LVFS、LVPWTd、LVPWTs低于假手术组,LVEDV、LVESV高于假手术组,差异均有统计学意义(P<0.01);生脉注射液组LVEF、LVFS、LVPWTd、LVPWTs高于模型组,LVEDV、LVESV低于模型组,差异有统计学意义(P<0.05或P<0.01)。模型组大鼠心肌细胞凋亡比率明显高于假手术组,生脉注射液组大鼠心肌细胞凋亡比率明显低于模型组,差异有统计学意义(P<0.01)。模型组大鼠心肌组织总SOD活力低于假手术组,生脉注射液组大鼠的心肌组织总SOD活力高于模型组,差异有统计学意义(P<0.05或P<0.01);模型组大鼠心肌组织总MDA含量高于假手术组,生脉注射液组大鼠的心肌组织总MDA含量低于模型组,差异有统计学意义(P<0.01)。模型组大鼠心肌组织Mfn2 mRNA表达和Mfn2蛋白表达均低于假手术组,生脉注射液组大鼠心肌组织Mfn2 mRNA表达和Mfn2蛋白表达均高于模型组,�Objective To investigate the effect and protective mechanism of Shengmai Injection on mitochondrial fusion factor 2(Mfn2)in myocardial ischemia reperfusion injury rats.Methods Eighteen Sprague-Dawley(SD)rats without specific pathogen(SPF)were randomly divided into sham operation group,model group,and Shengmai Injection group,with 6 rats in each group.The rat model of acute myocardial ischemia reperfusion Injury was established by ligation of the anterior descending branch of the left coronary artery for 30 minutes and reperfusion for 2 hours.The rats in sham operation group and model group were intraperitoneally injected with normal saline 12 mL/kg for 7 consecutive days,and Shengmai Injection group was Intraperitoneally injected with Shengmai Injection 12 mL/kg for 7 consecutive days before surgery.Left ventricular ejection fraction(LVEF),and left ventricular fraction shortening(LVFS),left ventricular posterior wall end-diastolic thickness(LVPWTd),and left ventricular posterior wall end-systolic thickness(LVPWTs),left ventricular end-diastolic volume(LVEDV),and left ventricular end-systolic volume(LVESV)were detected by ultrasound.TUNEL assay was used to detect myocardial cell apoptosis in rats.The activity of superoxide dismutase(SOD)and the content of malondialdehyde(MDA)were detected by fluorescence probe,the expression of Mfn2 mRNA was detected by reverse transcription polymerase chain reaction(RT-PCR),and the expression of Mfn2 protein and Caspase-3 protein were detected by Western Blot.Results LVEF,LVFS,LVPWTd,and LVPWTs in the rats of model group was lower than those in sham operation group,LVEDV and LVESV in the sham operation group were significantly higher than those in the sham operation group(P<0.01).LVEF,LVFS,LVPWTd and LVPWTs in the rats of Shengmai Injection group were higher than those in the model group,while LVEDV and LVESV were lower than those in the model group,and the difference was statistically significant(P<0.05 or P<0.01).The apoptosis ratio of myocardial cells in model group was Signif

关 键 词：心肌缺血再灌注损伤 生脉注射液 细胞凋亡 心肌细胞保护 实验研究 

分 类 号：R54[医药卫生—心血管疾病]