Rapid and sensitive analysis of trace b-blockers by magnetic solidphase extraction coupled with Fourier transform ion cyclotron resonance mass spectrometry  

作　　者：Xue Xiao Kaili He Ya-Jun Hou Zhangmin Xiang Yunyun Yang 

机构地区：[1]Guangdong Provincial Key Laboratory of Chemical Measurement and Emergency Test Technology,Guangdong Provincial Engineering Research Center for Ambient Mass Spectrometry,Institute of Analysis,Guangdong Academy of Sciences(China National Analytical Center,Guangzhou),Guangzhou,510070,China

出　　处：《Journal of Pharmaceutical Analysis》2022年第2期293-300,共8页药物分析学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(Grant Nos.:21976168,22127810,and 22004113);Key Research and Development Program of Guangdong Province(Grant No.:2020B1111350002);Guangdong Basic and Applied Basic Research Foundation(Grant No.:2019A1515110420);GDAS0 Project of Science and Technology Development(Grant No.:2021GDASYL-20210103034).

摘　　要：A rapid and sensitive method for analyzing trace b-blockers in complex biological samples,which involved magnetic solid-phase extraction(MSPE)coupled with Fourier transform ion cyclotron resonance mass spectrometry(FTICR-MS),was developed.Novel nanosilver-functionalized magnetic nanoparticles with an interlayer of poly(3,4-dihydroxyphenylalanine)(polyDOPA@Ag-MNPs)were synthesized and used as MSPE adsorbents to extract trace b-blockers from biological samples.After extraction,the analytes loaded on the polyDOPA@Ag-MNPs were desorbed using an organic solvent and analyzed by FTICR-MS.The method was rapid and sensitive,with a total detection procedure of less than 10 min as well as limits of detection and quantification in the ranges of 3.5-6.8 pg/mL and 11.7-22.8 pg/mL,respectively.The accuracy of the method was also desirable,with recoveries ranging from 80.9%to 91.0%following the detection of analytes in human blood samples.All the experimental results demonstrated that the developed MSPE-FTICR-MS method was suitable for the rapid and sensitive analysis of trace b-blockers in complex biological samples.

关 键 词：Β-BLOCKERS Magnetic solid-phase extraction Nanosilver-functionalized magnetic nanoparticles Poly(3 4-dihydroxyphenylalanine) Fourier transform ion cyclotron resonance mass spectrometry 

分 类 号：R962[医药卫生—药理学]