Phosphatidylethanolamine functionalized biomimetic monolith for immobilized artificial membrane chromatography  

作　　者：Peijie Zhu Weijia Chen Qiqin Wang Huihui Wu Meng Ruan Hongwu Wang Zhengjin Jiang 

机构地区：[1]School of Food&Pharmaceutical Engineering,Zhaoqing University,Zhaoqing,Guangdong,526061,China [2]Institute of Pharmaceutical Analysis,College of Pharmacy,Jinan University,Guangzhou,510632,China [3]School of Pharmaceutical Engineering,Guangdong Food and Drug Vocational College,Guangzhou,510632,China [4]Department of Pharmacy and Guangdong Province Key Laboratory of Pharmacodynamic Constituents of Traditional Chinese Medicine&New Drug Research,Jinan University,Guangzhou,510632,China

出　　处：《Journal of Pharmaceutical Analysis》2022年第2期332-338,共7页药物分析学报（英文版）

基　　金：funded by the National Natural Science Foundation of China(Grant Nos.:81872830 and 82073806);the Natural Science Foundation of Guangdong Province(Grant No.:2020A1515010569);the Science and Technology Innovation Guidance Project of Zhaoqing City(Grant No.:201804030103);the Scientific Research Fund of Zhaoqing University(Grant No.:201817).

摘　　要：In this research,a new phospholipid based monolith was fabricated by in situ co-polymerization of 1-dodecanoyl-2-(11-methacrylamidoundecanoyl)-sn-glycero-3-phosphoethanolamine and ethylene dimethacrylate to mimick bio-membrane environment.Excellent physicochemical properties of this novel monolith that were achieved included column efficiency,stability,and permeability.Moreover,the biomimetic monolith showed outstanding separation capability for a series of intact proteins and small molecules.In particular,it exhibited good potential as an alternative to the commercial immobilized artificial membrane(IAM)column(IAM.PC.DD2)for studying drug-membrane interactions.This study not only enriched the types of IAM stationary phases,but also provided a simple model for the prediction of phosphatidylethanolamine related properties of drug candidates.

关 键 词：PHOSPHATIDYLETHANOLAMINE Biomimetic monolith IAM Drug-membrane interaction 

分 类 号：R91[医药卫生—药学]