高效降解喹啉好氧颗粒污泥的快速形成及性能研究  

Rapid Formation and Performance of Aerobic Granular Sludge for Efficient Quinoline Degradation

作　　者：王佳璇 李政阳 刘喆[4] 宁方志 刘永军[4] WANG Jia-xuan;LI Zheng-yang;LIU Zhe;NING Fang-zhi;LIU Yong-jun(School of Architecture and Civil Engineering,Xi’an University of Science and Technology,Xi’an 710054,China;Post-doctoral Research Center of Mining Engineering,Xi’an University of Science and Technology,Xi’an 710054,China;Shaanxi Shanbei Mining Co.Ltd.,Yulin 719000,China;School of Environmental and Municipal Engineering,Xi’an University of Architecture and Technology,Xi’an 710055,China)

机构地区：[1]西安科技大学建筑与土木工程学院,陕西西安710054 [2]西安科技大学矿业工程博士后流动站,陕西西安710054 [3]陕西陕北矿业有限责任公司,陕西榆林719000 [4]西安建筑科技大学环境与市政工程学院,陕西西安710055

出　　处：《中国给水排水》2022年第9期8-17,共10页China Water & Wastewater

基　　金：国家自然科学基金资助项目(51808442、52170051、51808432);中国博士后科学基金资助面上项目(2020M683679XB);西安科技大学博士启动基金资助项目(2018QDJ023);陕西省自然科学基础研究计划项目(2018JQ5014、2019JQ-529);榆林市科技计划项目(2019-179、2019-136、CXY-2020-060、CXY-2020-101);陕西省高校科协青年人才托举计划项目(20200421)。

摘　　要：在SBR中利用污水厂活性污泥历经34 d快速培养出能高效降解喹啉的好氧颗粒污泥(AGS),其平均粒径为0.83 mm,MLSS为3.25 g/L,SVI30为36.92 mL/g,对喹啉的去除率为91.18%,并具有良好的同步脱氮除磷性能。在AGS的驯化期间,首次加入20 mg/L喹啉后,会在短期内造成出水水质与污泥性能的恶化,不过,在AGS强大的耐受性下,污泥各项指标均能快速恢复。当喹啉浓度提高至40 mg/L时,短期内去除效能会有所下降,但污泥表现出了良好的抗性,并随着颗粒化的加深对喹啉的去除性能进一步提高。喹啉会刺激污泥微生物分泌大量紧密结合型胞外聚合物(TBEPS),并以蛋白质(PN)为主,从而形成保护屏障以抵御外界胁迫作用。微生物高通量测序结果表明,成熟的AGS中具有反硝化及分泌EPS能力的Thauera、Zoogloea等功能菌群丰度较高,并且Acidovorax、Paracoccus等喹啉高效降解菌群得到了富集,从而形成了更加稳定的生态位。In sequencing batch reactor(SBR),activated sludge was used to rapidly cultivate aerobic granular sludge(AGS)with high efficiency for degrading quinoline after 34 days,with an average particle size of 0.83 mm,mixed liquor suspended solids(MLSS)of 3.25 g/L,and sludge volume index(SVI30)of 36.92 mL/g.For the mature AGS,the removal rate of quinoline was 91.18%,and it had excellent performance of simultaneous nitrogen and phosphorus removal.During the domestication of AGS,the first addition of 20 mg/L quinoline would cause the deterioration of effluent quality and sludge performance in a short period.Nevertheless,as the sludge adapted to quinoline,these indicators were recovered.When the concentration of quinoline was increased to 40 mg/L,the removal efficiency decreased in a short time,but sludge showed good resistance and with the deepening of granulation,the removal of quinoline was further improved.Additionally,quinoline stimulated sludge microorganisms to secrete a large amount of TB-EPS,mainly protein(PN),thus forming a protective barrier against external stress.High-throughput sequencing results showed that Thauera,Zoogloea and other functional bacteria with the ability for denitrifying and EPS secreting were highly abundant in mature AGS.Moreover,the efficient quinoline-degrading bacteria groups such as Acidovorax and Paracoccus were enriched,thus forming a more stable ecological niche.

关 键 词：好氧颗粒污泥 序批式反应器 喹啉 胞外聚合物 微生物群落 

分 类 号：TU992[建筑科学—市政工程]