不同佐剂伪狂犬病病毒主要抗原决定簇gBb亚单位疫苗免疫小鼠安全性及免疫效力测定  

Safety and efficacy evaluation of gBb subunit vaccine with different adjuvants for pseudorabies virus

作　　者：郭广君[1,2] 韩强 吕素芳 王文秀 李峰[1,2] 李振伟[2] 谢金文 张翠花 沈志强[1,2] GUO Guangjun;HAN Qiang;LYU Sufang;WANG Wenxiu;LI Feng;LI Zhenwei;XIE Jinwen;ZHANG Cuihua;SHEN Zhiqiang(Research and Development Center of Shandong Binzhou Animal Propolis Vaccine,Shandong Binzhou Animal Husbandry&Veterinary Medical Academy,Binzhou,Shandong 256600,China;Shandong LvDu Bio-Science Technology Co.,LTD,Binzhou,Shandong256600,China)

机构地区：[1]山东省滨州畜牧兽医研究院山东省畜禽用蜂胶疫苗研究开发推广中心,山东滨州256600 [2]山东绿都生物科技有限公司,山东滨州256600

出　　处：《中国兽医学报》2022年第3期426-431,共6页Chinese Journal of Veterinary Science

基　　金：山东省“外专双百计划”资助项目(WST2018014);山东省现代农业产业技术体系生猪创新团队资助项目(SDAIT-08-17)。

摘　　要：为了评价不同佐剂猪伪狂犬病病毒(PRV)SA株gB蛋白主要抗原决定簇gBb亚单位疫苗免疫小鼠的安全性和免疫效力,以PRV SA强毒株基因组为模板扩增gB基因保守区域,连接pET-28a载体构建pET-28a/gB表达质粒,并经基因测序和Western blot鉴定;制备的抗原与不同佐剂配比分别制备亚单位疫苗,开展不同佐剂gB蛋白主要抗原决定簇gBb亚单位疫苗免疫昆明小鼠免疫效力评估,测定免疫后不同时间体液免疫抗体水平,进行免疫攻毒保护试验,测定免疫保护力。结果表明,不同佐剂gB蛋白主要抗原决定簇gBb亚单位疫苗免疫昆明小鼠后能够产生较高的抗体水平,模式动物昆明小鼠攻毒免疫保护率达到60%,为PRV新型亚单位疫苗的研制提供了依据。In order to evaluate the safety and immune efficacy of the main epitope of the gB protein of pseudorabies virus(PRV) SA strain with different adjuvants in mice, the conserved region of gB gene was amplified using the genome of the PRV SA virulent strain as a template.The expression plasmid pET-28 a/gB was constructed, and the expression was identified by Western blot.Antigens were prepared and mixed with different adjuvants to prepare subunit vaccines, then the immune efficacy was evaluated by immunizing Kunming mice.The level of humoral immune antibodies at different times after immunization was determined, an immune challenge protection experiment was conducted to evaluate the immune protection effect.The results showed that the epitope vaccine of gB protein with different adjuvants could produce higher antibody levels after immunizing Kunming mice, and the protection rate of the model animal Kunming mice against challenge reached 60%.The research provided a basis for the development of a new type of PRV subunit vaccine.

关 键 词：PRV gB蛋白抗原决定簇gBb亚单位疫苗 疫苗佐剂 安全性 免疫效力 

分 类 号：S852.65[农业科学—基础兽医学]