地衣芽孢杆菌BL-24对烟草炭疽病的生物防治研究  

Study on the Biological Control of Bacillus licheniformis BL-24 to Tobacco Anthracnose

作　　者：原晨虹 任广伟[2] 张家韬 陈超 崔传斌 安德荣[1] YUAN Chenhong;REN Guangwei;ZHANG Jiatao;CHEN Chao;CUI Chuanbin;AN Derong(College of Plant Protection,Northwest A&F University,Yangling 712100;Tobacco Research Institute,CAAS,Qingdao 266000;Tobacco Research Institute of Shaanxi Province,Xi'an 710068,China)

机构地区：[1]西北农林科技大学植物保护学院,陕西杨凌712100 [2]中国农业科学院烟草研究所,山东青岛266000 [3]陕西省烟草科学研究所,西安710068

出　　处：《东北农业科学》2022年第2期75-78,共4页Journal of Northeast Agricultural Sciences

基　　金：中国烟草总公司重大专项[110201601023(LS-03)];陕西省绿色防控科技专项(KJ-2017-01)。

摘　　要：本试验研究地衣芽孢杆菌BL-24对烟草炭疽病的生物防治效果,用BL-24的发酵液与发酵上清液分别进行烟草炭疽病菌的平板对峙试验,结果显示菌丝生长抑制率分别为66.67%和68.89%。通过田间试验,分别采用地衣芽孢杆菌BL-24的发酵液、发酵上清液以及高温灭菌液与化学农药对比进行小区试验,结果显示,地衣芽孢杆菌BL-24发酵液较发酵上清液和高温灭菌液对烟草炭疽病的防治效果更加明显,防治效果达到56.28%,与化学药剂多菌灵的防治效果差异不显著,表明地衣芽孢杆菌BL-24是一株优良的微生物生防菌株,为烟草炭疽病的防治提供一条新的途径,也为地衣芽孢杆菌开发成生物制剂提供理论依据。In this study,the biological control effect of Bacillus licheniformis BL-24 on tobacco anthracnose was studied.The tabular confrontation test of tobacco anthrax was carried out with BL-24 fermentation broth and ferment supernatant respectively.The results showed that the mycelium growth inhibition rate was 66.67%and68.89%respectively.Through field experiment,cell experiments were carried out using fermentation broth,ferment supernatant,and high temperature sterilizing solution of B.licheniformis BL-24 in comparison with chemical pesticides.The results showed that the live bacteria of B.licheniformis BL-24 was more effective than the other two in the control of tobacco anthracnose,and the average control effect reached 56.28%.The difference between the control effect and the chemical agent carbendazim is not significant,indicating that B.licheniformis BL-24 is an excellent microbial biocontrol strain,which provides a new way for the prevention and control of tobacco anthracnose,and also provides a theoretical basis for the development of B.licheniformis biologics.

关 键 词：地衣芽孢杆菌BL-24 烟草炭疽病 生物防治 

分 类 号：S435.72[农业科学—农业昆虫与害虫防治]