葡萄籽-菊花-三七复合物的急毒、遗传毒性及长期毒性研究  

Study on Acute,Genetic and Long-Term Toxicities of Grape Seed-Chrysanthemum-Panax Notoginseng Complex

作　　者：张佳 杨静[1,2] ZHANG Jia;YANG Jing(School of Chemical Engineering,Research Institute,Northwest University,Xi′an 710069,China;Biotech.&Biomed.Research Institute,Northwest University,Xi′an 710069,China)

机构地区：[1]西北大学化工学院,陕西西安710069 [2]西北大学生物医药研究院,陕西西安710069

出　　处：《化学试剂》2022年第7期1028-1035,共8页Chemical Reagents

基　　金：国家重点研发计划项目(2021YFC2101500);中国博士后科学基金项目(2018M643720)。

摘　　要：以葡萄籽-菊花-三七复合物为研究对象,依据国家标准《食品安全性毒理学评价程序和方法》(GB 15193—2014)进行急毒、遗传毒性以及长期毒性评价,为三者复配的安全应用提供实验参考。结果表明,小鼠急性经口毒性试验中最大耐受剂量(MTD)>15.0 g/kg,属无毒级别。三项遗传学毒性试验结果显示均为阴性,没有发现致突变作用,说明葡萄籽-菊花-三七复合物无遗传学毒性。大鼠28 d喂养试验中,各剂量组(4.0、2.0和1.0 g/kg)试验动物生长状况良好,动物体重、动物进食量以及食物利用率、血液学和血清生化学、大鼠脏器系数等指标与空白对照组相比均无显著性差异,且大鼠组织经解剖检测均未见明显的病理组织学异常。综上所述,葡萄籽-菊花-三七复合物无可见毒副作用,具有较高的食用安全性。The toxicology and safety of grape seed,chrysanthemum,and notoginseng extracts were evaluated to provide the scientific basis for their safe combined applications.Acute oral,genetic,and long-term toxicity tests were performed according to the national standard of《Procedure and methods of food safety toxicological assessment》(GB 15193—2014).The maximum tolerated dose(MTD)in the acute oral toxicity test was more than 15.0 g/kg in mice,indicating grape seed-chrysanthemum-panax notoginseng complex was non-toxic.In the three genotoxicity tests of ames test,the micronucleus test of mice erythrocyte and mouse spermatocyte chromosomal aberration test,the results were all negative,and no mutagenic effect was found,indicating that grape seed-chrysanthemum-panax notoginseng complex has no genotoxicity.In the 28-day oral toxicity test,the rats in each group(4.0,2.0 and 1.0 g/kg)grew well,and there were no significant differences in body weight,food intake,food utilization efficiency,hematology and serum biochemistry,organ/body weight ratio comparing with the blank control group.And no obvious histopathological changes were found.All the toxicology data from this research verified that the grape seed-chrysanthemum-panax notoginseng complex was safe and non-toxic with good edible safety.

关 键 词：葡萄籽 菊花 三七 复配配方 毒理学 安全性 

分 类 号：R994.4[医药卫生—毒理学]