OMT对高交感活性诱导大鼠心室肥厚的保护作用  

Protective effect of OMT on ventricular hypertrophy induced by sympathetic overactivity in vivo

作　　者：王恒[1] 吉杨丹[1] 唐慧敏[1] 覃建锋[1] 梁子聪[1] 胡先运[1,2] 孟铁宏 WANG Heng;JI Yang-Dan;TANG Hui-Min(Qiannan Medical College for Nationalities,Duyun 558000,Guizhou,China)

机构地区：[1]黔南民族医学高等专科学校,贵州都匀558003 [2]贵州医科大学天然产物化学重点实验室

出　　处：《中国老年学杂志》2022年第13期3240-3243,共4页Chinese Journal of Gerontology

基　　金：国家自然科学基金项目(81860317);贵州省青年科技人才项目(黔教合KY字[2018]180号);贵州省中医药管理局中医药、民族医药科学技术研究项目(QZYY-2018-99);黔南科技计划基金资助项目(黔南科合社字[2017]74号);黔南民族医学高等专科学校校基金资助项目(QNYZ201713)。

摘　　要：目的研究氧化苦参碱(OMT)对高交感活性诱导大鼠实验性心室肥厚的改善作用。方法SD大鼠随机分为对照组、模型组、OMT高、低剂量组、普萘洛尔组,各组于造模前1 w开始灌胃给药,除对照组外,其余各组均连续腹腔注射去甲肾上腺素(NE)15 d复制实验性心肌损伤模型,造模结束后检测大鼠血压指标、取材检测心室重构参数及羟脯胺酸含量,qRT-PCR法检测Notch-1信号变化。结果OMT能够改善大鼠血压变化,降低心室重构参数及羟脯胺酸含量,改善Notch-1信号变化。结论OMT对高交感活性诱导的实验性心肌肥厚有显著的改善作用,可能与降低Notch-1信号的mRNA表达有关。Objective To investigate the ameliorated effect of oxymatrine(OMT)on experimental ventricular hypertrophy induced by high sympathetic activity in rats.Methods SD rats were divided into 5 groups as following:control group,model group,OMT high dose and low dose groups,propranolol group.Rats in each group were given the drug by intragastric administration 1 week before model establishment.The rat model of experimental myocardial hypertrophy was established by exposed to norepinephrine(NE)by intraperitoneal injection for 15 d in all groups except the control group.At the end of modeling,the blood pressure indexes,ventricular remodeling parameters and myocardial hydroxyproline content were determined after remodeling.Meanwhile,the Notch-1 signal was detected by qRT-PCR.Results OMT could improve blood pressure,reduce ventricular remodeling parameters and the content of hydroxyproline in myocardium of rats,improve Notch-1 signal changes.Conclusions OMT could significantly improve experimental myocardial hypertrophy induced by sympathetic overactivity,which is related to the reduction of Notch-1 signal mRNA expression.

关 键 词：OMT 高交感活性 心肌肥厚 Notch-1信号 

分 类 号：R965[医药卫生—药理学]