Modifying current thin-film microextraction(TFME)solutions for analyzing prohibited substances:Evaluating new coatings using liquid chromatography  

作　　者：Łukasz Sobczak Dominika Kołodziej Krzysztof Gorynski 

机构地区：[1]Bioanalysis Scientific Group,Faculty of Pharmacy,Collegium Medicum in Bydgoszcz at Nicolaus Copernicus University in Torun,85-089,Bydgoszcz,Poland

出　　处：《Journal of Pharmaceutical Analysis》2022年第3期470-480,共11页药物分析学报（英文版）

基　　金：supported by the National Centre for Research and Development under the Lider IX programme(Grant No.:LIDER/44/0164/L-9/17/NCBR/2018);Permission to conduct experiments with controlled substances was issued by the local Pharmaceutical Inspector(Kujawsko-Pomorski Wojewodzki Inspektor Farmaceutyczny w Bydgoszczy;Permission No.:WIFBY-KK.857.2.4.2016).

摘　　要：For identifying and quantifying prohibited substances,solid-phase microextraction(SPME)continues to arouse interest as a sample preparation method.However,the practical implementation of this method in routine laboratory testing is currently hindered by the limited number of coatings compatible with the ubiquitous high-performance liquid chromatography(HPLC)systems.Only octadecyl(C18)and polydimethylsiloxane/divinylbenzene ligands are currently marketed for this purpose.To address this situation,the present study evaluated 12 HPLC-compatible coatings,including several chemistries not currently used in this application.The stationary phases of SPME devices in the geometry of thin filmcoated blades were prepared by applying silica particles bonded with various functional ligands(C18,octyl,phenyl-hexyl,3-cyanopropyl,benzenesulfonic acid,and selected combinations of these),as well as unbonded silica,to a metal support.Most of these chemistries have not been previously used as microextraction coatings.The 48 most commonly misused substances were selected to assess the extraction efficacy of each coating,and eight desorption solvent compositions were used to optimize the desorption conditions.All samples were analyzed using an HPLC system coupled with triple quadrupole tandem mass spectrometry.This evaluation enables selection of the best-performing coatings for quantifying prohibited substances and investigates the relationship between extraction efficacy and the physicochemical characteristics of the analytes.Ultimately,using the most suitable coatings is essential for trace-level analysis of chemically diverse prohibited substances.

关 键 词：Sample preparation Solid-phase microextraction Thin-film microextraction Prohibited substances Drugs of abuse High-performance liquid chromatography 

分 类 号：R917[医药卫生—药物分析学]