抗TMV普通烟中N导入片段的特异分子标记开发与交换单株筛选  

The development of molecular markers of the N introgression segment in tobacco mosaic virus (TMV) resistant tobacco germplasms and exchange plant screening

作　　者：刘勇[1] 黄昌军[1] 陈姝敏 肖炳光[1] 于海芹[1] 袁诚 曾建敏[1] 曹培健[3] 李永平[1] LIU Yong;HUANG Changjun;CHEN Shumin;XIAO Binguang;YU Haiqin;YUAN Cheng;ZEN Jiagmin;CAO Peijian;LI Yongping(Yunnan Academy of Tobacco Agricultural Sciences,Key Laboratory of Tobacco Biotechnological Breeding,National Tobacco Genetic Engineering Research Center,Kunming 650021,China;Institute of Vegetables and Flowers,CAAS(Chinese Academy of Agricultural Sciences),Beijing 100081,China;Zhengzhou Tobacco Research Institute of CNTC,Zhengzhou 450001,Henan,China)

机构地区：[1]云南省烟草农业科学研究院/烟草行业烟草生物技术育种重点实验室/国家烟草基因工程研究中心,昆明650021 [2]中国农业科学院蔬菜花卉研究所,北京100081 [3]中国烟草总公司郑州烟草研究院,郑州450001

出　　处：《中国烟草学报》2022年第3期89-95,共7页Acta Tabacaria Sinica

基　　金：中国烟草总公司云南省公司科技项目“利用短N导入片段材料定向改良云烟87品种TMV抗性”(2018530000241003)。

摘　　要：【背景和目的】N导入片段赋予烟草品种对烟草花叶病毒(Tobacco mosaic virus,TMV)抗性,但同时带来产量低等连锁累赘,为了筛选减少连锁累赘的交换单株。【方法】以普通烟草中的N导入片段为材料,利用茄科作物基因组序列数据和比较基因组学方法,开发了特异扩增N导入片段的系列分子标记,通过分子标记在种质资源和分离群体中筛选交换单株。【结果】开发出23个N导入片段特异的分子标记,将携带N导入片段的普通烟种质划分为Coker176、Samsun NN和Xanthi nc三种长度类型。供试90份抗TMV的普通烟资源中,85份属于N导入片段长度相对较短的Coker176类型,未发现比Coker176更短的种质资源。利用N导入片段末端分子标记TN5.51,从不含N基因的K326、Y87、5F、HD与Coker176配置的4个回交分离群体的22572株抗TMV单株中,筛选到11株N导入片段交换单株,Coker176类型的N导入片段在普通烟中的交换频率为0.049%。其中24-8H等3个单株的N导入片段缩短约一半,具有减少连锁累赘的潜力。【结论】本研究获得了普通烟中N导入片段的系列分子标记和长度缩短一半的交换单株,为解析N导入片段连锁累赘的分子机理、减少连锁累赘奠定了基础。Many good disease-resistant genes in Solanaceae crop cultivars come from the chromosome introgression segment of wild species, but the non-target genome sequences on the exogenous chromosomes introgression segment may cause linkage drag, which limits the utilization of good genes of wild species. One of the well-known exogenous chromosome introgression segment in crop breeding is the N gene(N introgression segment) in Nicotiana tabacum introgressed from N. glutinosa. The N introgression segment endows resistance against tobacco mosaic virus(TMV) while causing linkage drag in tobacco leaf production. [Objective] To screen short N introgression segment exchange plant, [Methods] molecular markers specific to the N introgression segment were developed using the tomato genome as reference. [Results] A total of 23 N introgression segment specific molecular markers were developed. The markers are located in 3.50Mb~ 5.77Mb of chromosome 11 of tomato reference genome. Using five N introgression segment specific markers and one N.tabacum specific markers, three length types of introgression segments were distinguished, named Coker176, Samsun NN and Xanthi nc. Eighty-five from95 tested N.tabacum germplasms had relatively short introgression segments, which belong to the Coker176 type. No germplasm carrying shorter N segment than the Coker176 was found. Using the introgression segment end molecule mark TN5.51, 11 exchange plants were screened from 22572 plants from 4 separation groups inoculated with TMV to show necrotic lesion. The N introgression segment of the Coker176 length type has an average exchange frequency of 0.049% in N.tabacum. Among them, the N introgression segments of 24-8H and other 2 exchange plants were shortened by about half, showing the potential to reduce the linkage drags. [Conclusion] The series molecular markers specific to the N introgression segment and the exchange plants with half N introgression segment were developed,which provides a basis for analyzing the molecular mechanism of linkage drag

关 键 词：普通烟草 N导入片段 分子标记 交换单株 

分 类 号：S435.72[农业科学—农业昆虫与害虫防治]