红树林内生真菌Aspergillus fumigatus SAS10二聚萘并吡喃酮类代谢产物研究  

Dimeric naphthopyrones produced by a mangrove endophytic fungus Aspergillus fumigatus SAS10

作　　者：陈赞鸿 朱静琳 林灵茵 郭思雨 黄美荧 徐文艳 黄洪波 陶移文 CHEN Zan-hong;ZHU Jing-lin;LIN Ling-yin;GUO Si-yu;HUANG Mei-ying;XU Wen-yan;HUANG Hong-bo;TAO Yi-wen(School of Pharmaceutical Sciences,Guangzhou Medical University;Key Laboratory of Molecular Target&Clinical Pharmacology,Guangzhou 511436,China)

机构地区：[1]广州医科大学药学院 [2]广东省分子靶标与临床药理学重点实验室,广州511436

出　　处：《天然产物研究与开发》2022年第6期967-974,共8页Natural Product Research and Development

基　　金：广州医科大学学生科技创新项目(2020A068);广东省科技创新战略专项(大学生科技创新培育)项目(pdjh2021b0417);国家大学生创新创业训练项目(202110570035);广州市教育局高校“羊城学者”科研项目(202032774)。

摘　　要：通过大米培养基静置发酵无瓣海桑内生真菌Aspergillus fumigatus SAS10,采用硅胶柱色谱、Sephadex LH-20凝胶柱色谱以及半制备高效液相色谱等分离手段对其发酵产物进行分离纯化,获得8个纯化合物,其结构经核磁共振波谱、质谱等现代分析方法,分别鉴定为asperpyrone A(1)、aurasperone B(2)、asperpyrone C(3)、asperpyrone D(4)、dianhydro-aurasperone C(5)、aurasperone A(6)、fonsecinone A(7)和rubrofusarin B(8)。结构鉴定结果显示,化合物1~8均为首次从烟曲霉中分离得到,菌株Aspergillus fumigatus SAS10具有产生大量二聚萘并吡喃酮类代谢产物的潜力。抑菌活性表明,化合物1~8对金黄色葡萄球菌、大肠杆菌和耐甲氧西林金黄色葡萄球菌具有不同程度的抑制活性。The mangrove endophytic fungus Aspergillus fumigatus SAS10 from Sonneratia apetala was fermented by using rice medium,and eight compounds were obtained by isolation and purification of metabolites with modern separation techniques such as column chromatography on silica gel,column chromatography on Sephadex LH-20,and semi-preparative HPLC.On the basis of mass and NMR spectroscopic data,their structures were identified as asperpyrone A(1),aurasperone B(2),asperpyrone C(3),asperpyrone D(4),dianhydro-aurasperone C(5),aurasperone A(6),fonsecinone A(7)and rubrofusarin B(8),respectively.All of compounds were reported from the mangrove endophytic fungus Aspergillus fumigatus for the first time,and Aspergillus fumigatus SAS10 is capable of producing a large number of dimeric naphthopyrones metabolites.The antimicrobial activities showed that all compounds exhibited different degrees of inhibitory activities against Staphylococcus aureus,Escherichia coli and Methicillin-resistant Staphylococcus aureus.

关 键 词：红树林 内生真菌 Aspergillus fumigatus 次级代谢产物 二聚萘并吡喃酮类化合物 

分 类 号：R284.1[医药卫生—中药学]