NaCl处理下全缘冬青和红果冬青根系的转录组活性比较  

Comparison of transcriptomic activity of Ilex integra and I. purpurea roots with NaCl treatments

作　　者：张强 周鹏[2] 刘昌来 余永帆 张敏[2] 杨甲定 ZHANG Qiang;ZHOU Peng;LIU Changlai;YU Yongfan;ZHANG Min;YANG Jiading(Co-Innovation Center for Sustainable Forestry in Southern China,College of Biology and the Environment,Nanjing Forestry University,Nanjing 210037,China;Jiangsu Academy of Forestry,Nanjing 211153,China)

机构地区：[1]南京林业大学,南方现代林业协同创新中心,南京林业大学生物与环境学院,江苏南京210037 [2]江苏省林业科学研究院,江苏南京211153

出　　处：《南京林业大学学报（自然科学版）》2022年第3期99-108,共10页Journal of Nanjing Forestry University：Natural Sciences Edition

基　　金：江苏省林业科技创新与推广项目(LYKJ[2021]07);江苏省林业科学研究院自主科研项目(ZZKY202105)。

摘　　要：【目的】研究全缘冬青(Ilex integra)和红果冬青(I. purpurea)的根系在NaCl胁迫处理中的转录组活性差异,为进一步研究二者耐盐性差异的生理学机理奠定基础。【方法】以内含250 mmol/L NaCl的1/2浓度霍格兰德溶液对全缘冬青和红果冬青的两年生扦插苗进行盐胁迫处理,采集经0(盐处理前)、6和72 h盐处理的根系样品,提取总RNA,进行转录组测序,组装转录本,筛选差异表达基因,分析两种冬青根系中的功能富集通路和特定代谢途径的基因表达变化。【结果】全缘冬青根系盐处理6 h的差异表达基因数量为2 616,盐处理72 h为1 802;而红果冬青根系盐处理6 h的差异表达基因数量为1 831,盐处理72 h为3 490。全缘冬青盐处理6和72h根系中共同的KEGG富集途径为植物激素信号转导、亚油酸代谢、类胡萝卜素生物合成和甜菜红素生物合成,红果冬青根系6和72 h盐处理共同的KEGG富集途径为植物激素信号转导、苯丙烷生物合成和类胡萝卜素生物合成。甜菜红素生物合成和亚油酸/花生四烯酸代谢为盐胁迫处理全缘冬青根系中特有的富集代谢通路。全缘冬青中受盐胁迫正调控的过氧化氢酶基因数多于红果冬青的,并且在盐处理下其表达水平远高于红果冬青的。【结论】全缘冬青根系较强的耐盐性可能与植物激素信号转导、类胡萝卜素生物合成、甜菜红素生物合成和脂肪酸脂类代谢等生理过程相关联;盐处理下过氧化氢酶基因的高效表达也可能是全缘冬青根系具较强耐盐性的因素之一。【Objective】 This research aims to investigate the difference in transcriptomic activity ofIlex integraandI.purpurearoots using NaCl salt treatments and provide insights for future study on the physiological mechanismsresponsible for differential salt tolerance between theseIlexspecies. 【 Method】 Two-year-old cutting seedlings ofI.integraandI. purpureawere irrigated with half-strength Hoagland solution containing 250 mmol/L NaCl for salttreatments. Root samples were collected at 0(before salt treatment), 6 and 72 h after salt treatments. The total RNAswere extracted for transcriptomic RNA-Seq. Transcripts were assembled and differentially expressed genes(DEGs) wereselected. The enriched KEGG functional pathways for the two species were compared, and the expression of genesinvolved in certain metabolic pathways was analyzed. 【Result】 There were 2 616 and 1 802 DEGs in 6 h-salt-treated and 72 h-salt-treatedI. integraroots respectively, while there were 1 831 and 3 490 DEGs in 6 h-salt-treated and 72 h-salt-treatedI. purpurearoots, respectively. The common enriched KEGG functional pathways betweenI. integraroots with 6and 72 h salt treatments were plant hormone signal transduction, linoleic acid metabolism, carotenoid biosynthesis andbetalain biosynthesis. The common enriched pathways betweenI. integraroots with 6 and 72 h salt treatments were planthormone signal transduction, phenylpropanoid biosynthesis and carotenoid biosynthesis. Betalain biosynthesis and linoleicacid/arachidonic acid metabolism are unique pathways inI. integraroots during salt treatments. There were more up-regulated putative catalase genes with much higher expression levels in salt-treatedI. integraroots than that in theI.purpurearoots. 【Conclusion】 The higher salt tolerance ofI. integraroots may be associated with physiological processessuch as plant hormone signal transduction, carotenoid biosynthesis, betalain biosynthesis and fatty acid metabolism. Thehigher expression of catalase genes during salt treatments may also contri

关 键 词：全缘冬青 红果冬青 根系 NaCl盐胁迫 转录组分析 代谢途径 

分 类 号：Q945[生物学—植物学] S718[农业科学—林学]