丹参多酚酸盐治疗慢性心力衰竭后外周血单核细胞Toll样受体4/核转录因子-κB信号通路变化观察  

Changes of TLR4/NF-κB signaling pathway in patients with chronic heart failure treated with salvia miltiorrhiza polyphenol

作　　者：李广鹏[1] 张建新[1] 李闯[1] 石金河[1] LI Guangpeng;ZHANG Jianxin;LI Chuang;SHI Jinhe(Department of Emergency,The First Affiliated Hospital of Xinxiang Medical University,Xinxiang 453100,China)

机构地区：[1]新乡医学院第一附属医院急救医学科,453100

出　　处：《心肺血管病杂志》2022年第5期480-484,491,共6页Journal of Cardiovascular and Pulmonary Diseases

摘　　要：目的:探讨丹参多酚酸盐治疗慢性心力衰竭(CHF)后外周血单核细胞Toll样受体4/核转录因子-κB(TLR4/NF-κB)信号通路变化情况。方法:选取2016年11月至2020年1月我院收治的CHF患者172例,采用随机数表法分为对照组和观察组,每组86例。对照组患者给予常规治疗,观察组患者在对照组治疗基础上给予丹参多酚酸盐治疗,两组患者均持续治疗2周。比较两组患者治疗前后超声参数(包括LVEF、LVEDD、LVESD)、6分钟步行距离(6MWD),分析两组患者治疗前后TNF-α、IL-1β、IL-6、TLR4、NF-κB mRNA和蛋白表达,并观察两组患者临床疗效。结果:两组患者治疗后LVEF显著高于治疗前(P<0.05),LVEDD、LVESD显著低于治疗前(P<0.05);观察组患者治疗后LVEF显著高于对照组(P<0.05),LVEDD、LVESD显著低于对照组(P<0.05)。两组患者治疗后TNF-α、IL-1β、IL-6 mRNA和蛋白表达显著低于治疗前(P<0.05);观察组患者治疗后TNF-α、IL-1β、IL-6 mRNA和蛋白表达显著低于对照组(P<0.05)。两组患者治疗后TLR4、NF-κB mRNA和蛋白表达显著低于治疗前(P<0.05);观察组患者治疗后TLR4、NF-κB mRNA和蛋白表达显著低于对照组(P<0.05)。观察组患者临床疗效优于对照组(P<0.05)。结论:丹参多酚酸盐治疗CHF临床疗效确切,能有效抑制外周血单核细胞TLR4/NF-κB信号通路,减轻炎性反应,改善心功能。Objective:To investigate the changes of toll-like receptor 4/nuclear factor-κB(TLR4/NF-κB)signaling pathway in peripheral blood monocytes after treatment of chronic heart failure(CHF)with salvia miltiorrhiza polyphenol.Methods:172 patients with CHF in our hospital from November 2016 to January 2020 were selected and randomly divided into control group and observation group,86 cases in each group.The control group was given conventional treatment,while the observation group was given salvianolate on the basis of the control group,both groups were treated for 2 weeks.Comparison of two groups before and after treatment in patients with ultrasound parameters(including LVEF,LVEDD,LVESD),6 minutes walking distance(6 MWDS),analysis of the two groups before and after treatment in patients with TNF-α,IL-1β,IL-6,TLR4,NF-κB mRNA and protein expression,and observe the clinical efficacy of two groups of patients.Results:LVEF of the two groups after treatment was significantly higher than that before treatment(P<0.05),LVEDd and LVESD were significantly lower than those before treatment(P<0.05);LVEF of the observation group was significantly higher than that of the control group(P<0.05),LVEDd and LVESD were significantly lower than those of the control group(P<0.05).The expressions of TNF-α,IL-1β,IL-6 mRNA and protein in the two groups after treatment were significantly lower than those before treatment(P<0.05);The expressions of TNF-α,IL-1β,IL-6 mRNA and protein in the observation group were significantly lower than those in the control group(P<0.05).The expression of TLR4 and NF-κB mRNA and protein in the two groups after treatment were significantly lower than those before treatment(P<0.05);The expression of TLR4 and NF-κB mRNA and protein in the obefore treatment(P<0.05);The expression of TLR4 and NF-κB mRNA and protein in the observation group were significantly lower than those in the control group(P<0.05).The clinical efficacy of the observation group TLR4/NF-κB signaling pathway in peripheral blood monocyte

关 键 词：丹参多酚酸盐 慢性心力衰竭 TOLL样受体4 核转录因子-ΚB 

分 类 号：R54[医药卫生—心血管疾病]