通体结香技术处理30 d产沉香的品质监测  

Quality Monitoring of Agarwood Produced by Whole-tree Agarwood-inducing Technique (Agar-WIT) within 30 Days

作　　者：何欣 张燕 孟慧 魏建和[1,2] 吕菲菲 刘培卫[2] 李浩凌[2] 陈波 黄良明 杨云[1,2] HE Xin;ZHANG Yan;MENG Hui;WEI Jian-he;LYU Fei-fei;LIU Pei-wei;LI Hao-ling;CHEN Bo;HUANG Liang-ming;YANG Yun(Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine,Ministry of Education&National Engineering Laboratory for Breeding of Endangered Medicinal Materials,Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences&Peking Union Medical College,Beijing 100193,China;Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine&Key Laboratory of State Administration of Traditional Chinese Medicine for Agarwood Sustainable Utilization,Hainan Branch,Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences&Peking Union Medical College,Haikou 570311,China)

机构地区：[1]中国医学科学院北京协和医学院药用植物研究所中草药物质基础与资源利用教育部重点实验室/濒危药材繁育国家工程实验室,北京100193 [2]中国医学科学院北京协和医学院药用植物研究所海南分所海南省南药资源保护与开发重点实验室/国家中医药管理局沉香可持续利用重点研究室,海南海口570311

出　　处：《中国现代中药》2022年第6期1074-1082,共9页Modern Chinese Medicine

基　　金：国家重点研发计划项目(2018YFC1706401)。

摘　　要：目的:研究通体结香技术诱导沉香形成的作用过程。方法:根据《中华人民共和国药典》2020年版沉香质量标准,对通体结香技术处理30 d内产生的沉香样品进行性状、沉香四醇质量分数、结香面积比率、特征图谱分析,并对部分特征型倍半萜类和芳香类化合物进行含量测定。结果:通体结香技术处理白木香第4~5天,木间韧皮部和射线细胞出现树脂类物质,形成环形沉香层,沉香结香率由4.31%急剧上升至8.11%。结香30 d可形成明显的沉香层结构,薄层色谱和特征图谱结果与对照药材一致。沉香四醇质量分数在结香30 d内由0.001%增长至0.034%,整体呈上升趋势。部分芳香类化合物和沉香特征性倍半萜类化合物分别在第1~8天和第9~16天开始产生并累积。结论:通体结香技术处理30 d能够诱导沉香快速形成和稳定积累。Objective:To investigate the process of agarwood produced by whole-tree agarwood-inducing technique(Agar-WIT).Methods:The characteristic,mass fraction of agarotetrol,agarwood area ratio and characteristic chromatogram of agarwood produced by Agar-WIT within 30 days were analyzed against the standards in Chinese Pharmacopeia(2020edition),and the content of some characteristic sesquiterpenoids and aromatic compounds was determined.Results:On the4thto 5thday after the treatment,resinous substance appeared in the interxylary phloem and rays of the agarwood,forming an annular agarwood layer,and the agarwood formation rate increased sharply from 4.31% to 8.11%.A clear agarwood layer structure can be formed within 30 days of treatment,and the results of thin-layer chromatography(TCL) and characteristic chromatogram of agarwood were consistent with the reference medicinal material.The mass fraction of agarotetrol increased from 0.001% to 0.034% within 30 days,showing an upward trend as a whole.Some aromatic compounds and characteristic sesquiterpenoids of agarwood began to form and accumulate in the 1stto 8thday and 9thto 16thday,respectively.Conclusion:Agar-WIT can promote the rapid formation and stable accumulation of agarwood within 30 days.

关 键 词：沉香 通体结香技术 动态监测 形成规律 

分 类 号：R282.2[医药卫生—中药学]