参芪颗粒对腺嘌呤致大鼠慢性肾功能衰竭模型的作用研究  

The Effect of Shenqi Granules on Adenine-induced Chronic Kidney Failure in Rats

作　　者：杨振[1] 李会影[1] 刘建璇[1] 于映霞 荆涛 YANG Zhen;LI Huiying;LIU Jianxuan;YU Yingxia;JING Tao(Academy of Traditional Chinese Medicine,Jilin Province,Changchun 130021,China;不详)

机构地区：[1]吉林省中医药科学院,吉林长春130021

出　　处：《中国医学创新》2022年第18期7-11,共5页Medical Innovation of China

基　　金：吉林省科技发展计划项目(20200404071YY)。

摘　　要：目的:研究参芪颗粒对慢性肾功能衰竭模型大鼠的保护作用,为新药开发提供药效学依据。方法:选取65只雄性SD大鼠,除空白对照组(n=12)外,其余大鼠前14 d,每天灌胃腺嘌呤混悬液150 mg/kg;后7 d,每天灌胃腺嘌呤混悬液200 mg/kg,以制作慢性肾衰大鼠模型。将造模成功的50只大鼠随机分为5组,即模型对照组,参芪颗粒高剂量组、中剂量组、低剂量组,阳性对照组,每组10只。观察参芪颗粒对CRF模型大鼠的保护作用。比较六组大鼠24 h尿量、24 h尿蛋白、血清肌酐(Scr)、血清尿素氮(BUN)、尿酸(UA)、体重、肾脏重量、肾脏指数。结果:模型对照组大鼠的24 h尿量多于空白对照组,Scr、BUN、UA水平均高于空白对照组,差异均有统计学意义(P<0.001)。模型对照组24 h尿蛋白量高于空白对照组,差异有统计学意义(P<0.01)。参芪颗粒高、中、低剂量组大鼠的24 h尿蛋白量均低于模型对照组,差异均有统计学意义(P<0.05)。参芪颗粒高剂量组Scr水平低于模型对照组,差异有统计学意义(P<0.001)。参芪颗粒高剂量组BUN水平低于模型对照组,差异有统计学意义(P<0.01)。参芪颗粒高剂量组UA水平低于模型对照组,差异有统计学意义(P<0.05)。参芪颗粒中剂量组和阳性对照组的Scr、BUN、UA均低于模型对照组,差异均有统计学意义(P<0.05)。参芪颗粒低剂量组UA水平低于模型对照组,差异有统计学意义(P<0.05)。模型对照组体重低于空白对照组,差异有统计学意义(P<0.01)。模型对照组肾脏重量、肾脏指数均高于空白对照组,差异均有统计学意义(P<0.001)。参芪颗粒高剂量组肾脏重量低于模型对照组,差异有统计学意义(P<0.01)。阳性对照组的肾脏重量、肾脏指数均高于模型对照组,差异均有统计学意义(P<0.05)。结论:参芪颗粒具有一定的改善肾功能,保护肾组织的作用,值得进一步开发利用。Objective:To study the protective effect of Shenqi Granules on chronic kidney failure rats,and to provide pharmacodynamic basis for new drug development.Method:A total of 65 male SD rats were selected,except the blank control group(n=12),the other rats were given intragastric administration of Adenine Suspension 150 mg/kgdaily for the first 14 d and 200 mg/kg daily for the next 7 d,to make a rat model of chronic renal failure.50 rats successfully modeled were randomly divided into 5 groups,model control group,Shenqi Granules high-dose group,medium-dose group,low-dose group and positive control group,with 10 rats in each group.The protective effect of Shenqi Granules on CRF model rats was observed.The 24 h urine volume,24 h urine protein,serum creatinine(Scr),serum urea nitrogen(BUN),uric acid(UA),body weight,kidney weight and renal index of rats were compared in six groups.Result:The 24 h urine volume of the model control group was higher than that of the blank control group,the levels of Scr,BUN and UA of the model control group were higher than those of the blank control group,the differences were statistically significant(P<0.001).The 24 h urinary protein level of the model control group was higher than that of the blank control group,the difference was statistically significant(P<0.01).The 24 h urinary protein content of Shenqi Granules high-dose,medium-dose and low-dose groups were lower than those of the model control group,the differences were statistically significant(P<0.05).Scr level of the Shenqi Granules high-dose group was lower than that of the model control group,the difference was statistically significant(P<0.001).The BUN level of the Shenqi Granules high-dose group was lower than that of the model control group,the difference was statistically significant(P<0.01).UA level of the Shenqi Granules high-dose group was lower than that of the model control group,the difference was statistically significant(P<0.05).Scr,BUN and UA of Shenqi Granules medium-dose group and positive control group were lowe

关 键 词：参芪颗粒 腺嘌呤 慢性肾功能衰竭 

分 类 号：R285.5[医药卫生—中药学]