Study on Tissue Culture and Rapid Propagation of Tilia amurensis  

作　　者：Yinhua WANG Yuguang KONG Yingjun HE Liping YAN Dejun WU 

机构地区：[1]Shandong Academy of Forestry Sciences/Shandong Key Laboratory of Forest Tree Genetic Improvement,Jinan 250014,China [2]Shandong Territorial and Spatial Planning Institute,Jinan 250014,China [3]Laoshan Nature Reserve,Yantai 264100,China

出　　处：《Agricultural Biotechnology》2022年第3期18-21,28,共5页农业生物技术（英文版）

基　　金：Supported by Key R&D Program of Shandong Province(2017GNC10112)。

摘　　要：To explore the establishment of a tissue culture and rapid propagation system of Tilia amurensis,the effects of basic medium and concentrations and ratios of plant growth regulators on tissue culture and rapid propagation of T.amurensis were studied.The results showed that 1/2 MS medium was the most suitable proliferation medium,and the proliferation coefficient could reach 13.5 after adding 0.05 mg/L 6-BA and 0.03 mg/L IBA;MS medium was the most suitable medium for strong plantlets and rooting,and the best medium for strong plantlets was MS+0.1 mg/L 6-BA+0.1 mg/L IBA+0.03 mg/L GA_(3),with which the average plantlet height reached 5.15 cm;and the best rooting medium was MS+1.0 mg/L6-BA+0.05 mg/L NAA,with which the rooting rate was 93.3%and the number of roots was 5.7 roots.

关 键 词：Tilia amurensis Tissue culture Medium for strong plantlets Medium for rooting Vegetative propagation Rapid propagation 

分 类 号：S687[农业科学—观赏园艺]