miR-155在银屑病皮肤间充质干细胞外泌体中的差异表达研究  

Differential expression study of miR-155 in exosomes of mesenchymal stem cells from psoriatic skin

作　　者：刘佳[1] 赵新程[1] 韩齐心 侯慧 牛旭平[1] LIU Jia;ZHAO Xin-cheng;HAN Qi-xin(Taiyuan Central Hospital Affiliated to Shanxi Medical University,Taiyuan 030009,China)

机构地区：[1]山西医科大学附属太原市中心医院,030009

出　　处：《中国实用医药》2022年第11期186-190,共5页China Practical Medicine

摘　　要：目的 使用实时荧光定量聚合酶链式反应(RT-qPCR)法检测来源于银屑病和健康人皮肤间充质干细胞(MSCs)外泌体中miR-155的差异表达情况。方法 分离培养3例银屑病患者皮损(银屑病组)及3例健康人皮肤(正常组)的MSCs,将两组各自细胞上清用超高速离心法提取外泌体,RT-qPCR检测miR-155表达水平。结果 银屑病组皮肤MSCs外泌体中miR-155的表达与正常组相比,外参检测正常,但两组样本中has-miR-155-5p均未测得,差异无统计学意义(P>0.05)。结论 银屑病患者皮肤MSC分泌的外泌体可能通过miR-155以外的其他小RNA参与银屑病的发病。Objective To study the differential expression of miR-155 in exosomes of mesenchymal stem cells(MSCs) from psoriatic skin. Methods MSCs were isolated and cultured from the skin lesions of 3 cases of psoriasis patients(psoriasis group) and 3 cases of healthy human skin(mormal group). Exosomes were extracted from the supernatants of the respective cells of both groups by ultra-high speed centrifugation,and miR-155 expression levels were detected by quantitative real-time polymerase chain reaction(RT-qPCR).Results Compared with the normal group, the expression of miR-155 in exosomes of skin MSCs in the psoriasis group was normal, but has-miR-155-5p was not detected in the two groups of samples, and the difference was not statistically significant(P>0.05). Conclusion Exosomes secreted by skin MSC of psoriasis patients may be involved in the pathogenesis of psoriasis through small RNAs other than miR-155.

关 键 词：银屑病 皮肤间充质干细胞 外泌体 MIR-155 异质核核糖核蛋白A2B1 

分 类 号：R758.63[医药卫生—皮肤病学与性病学]