胡桃醌对口腔鳞癌Tac8113细胞增殖与凋亡的作用及机制探究  

Effect and Mechanism of Walnut Quinone on Proliferation and Apoptosis of Oral Squamous Cell Carcinoma Tac8113 Cells

作　　者：李琨[1] 张华湘 董素阁[1] 崔丽丽 刘蕾[1] 李婷婷[1] LI Kun;ZHANG Huaxiang;DONG Suge;CUI Lili;LIU Lei;LI Tingting(The Fifth Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,Henan,China;Stomatological Hospital,Wuhan University,Wuhan 430079,Hebei,China)

机构地区：[1]郑州大学第五附属医院,河南郑州450052 [2]武汉大学口腔医院,湖北武汉430079

出　　处：《中华中医药学刊》2022年第4期107-111,I0016,共6页Chinese Archives of Traditional Chinese Medicine

基　　金：国家中医药管理局国家中医临床研究基地业务建设专项(JDZX20181573);河南省高等学校重点科研项目(教育厅)(14A320020)。

摘　　要：目的探究胡桃醌对口腔鳞癌Tac8113细胞增殖与凋亡的作用及机制。方法胡桃醌5、15、25、35、40μmol/L处理口腔鳞癌Tac8113细胞24、48、72 h,采用倒置显微镜观察细胞形态学变化,MTT检测细胞增殖情况,流式细胞术检测细胞凋亡率和细胞周期,蛋白免疫印迹检测B淋巴细胞瘤-2基因(Bcl-2)、Caspase-3、Caspase-9蛋白表达。结果胡桃醌组5、15μmol/L处理24 h后与对照组相比,无明显变化,处理48、72 h后,15、25、35、40μmol/L浓度可见细胞间连接松解,贴壁细胞皱缩变小或变圆,折光性强,呈多形性,并有漂浮的死细胞,细胞分裂相减少;同一时间点,胡桃醌组各浓度OD值与对照组相比,差异具有统计学意义(P<0.05);胡桃醌组不同浓度相同时间点OD值及相同浓度不同时间点OD值比较,差异均具有统计学意义,伴随胡桃醌浓度不断增大,对口腔鳞癌Tac8113细胞抑制率呈明显上升趋势(P<0.05);除5μmol/L 72 h外,同一时间点,胡桃醌组各浓度凋亡率与对照组相比均显著增加,差异具有统计学意义(P<0.05);胡桃醌组不同浓度相同时间点凋亡率及相同浓度不同时间点凋亡率比较,差异均具有统计学意义,伴随胡桃醌浓度不断增大,口腔鳞癌Tac8113细胞凋亡率呈明显上升趋势(P<0.05);同一时间点,胡桃醌各浓度组与对照组比较,G_(0)/G_(1)期细胞数减少,S期细胞数增加,G_(2)/M期细胞数减少(P<0.05);胡桃醌组不同作用浓度同一时间点细胞周期及同一浓度不同时间点,G_(0)/G_(1)期细胞数减少,S期细胞数增加,G_(2)/M期细胞数减少(P<0.05);同一时间点,胡桃醌各浓度组与对照组比较,Bcl-2降低,Caspase-3、Caspase-9升高(P<0.05);胡桃醌组不同作用浓度同一时间点及同一浓度不同时间点Bcl-2呈降低趋势,Caspase-3、Caspase-9呈升高趋势(P<0.05)。结论胡桃醌能抑制口腔鳞癌Tac8113细胞增殖,并促进凋亡,其机制与阻滞细胞周期于S期、上调促凋亡因子表达,下调抑制凋Objective To investigate the effect and mechanism of walnut quinone on the proliferation and apoptosis of oral squamous cell carcinoma Tac8113 cells.Methods Walnut quinone 5,15,25,35 and 40μmol/L were used to treat oral squamous cell carcinoma Tac8113 cells for 24 h,48 h and 72 h.The cellular morphological changes were observed with an inverted microscope.MTT was used to detect cell proliferation.The flow cytometry was used to detect apoptotic rate and cell cycle,and Western blot was used to detect B lymphoblastoma-2 gene(Bcl-2),Caspase-3 and Caspase-9 protein expressions.Results There were no significant changes in the walnut quinone group compared with the control group after treated with 5,15μmol/L for 24 h,48 h and 72 h treatment.The intercellular junctions were loosened at the concentrations of 15,25,35 and 40μmol/L,and the adherent cells shrunk to become smaller or rounder,which was highly refractive and polymorphic,and there were floating dead cells,and the cell division phase was reduced.At the same time point,the OD values of each concentration in the walnut quinone group were significantly different from those in the control group(P<0.05).The difference in OD values at the same time point and different time points at the same concentration in the walnut quinone group was statistically significant,with the increasing concentration of walnut quinone,the inhibition rate of oral squamous cell carcinoma Tac8113 cells showed a significant upward trend(P<0.05).Except for 5μmol/L for 72 h,at the same time point,the apoptosis rate of each concentration in the walnut quinone group increased significantly compared with that of the control group,and the difference was statistically significant(P<0.05).In the walnut quinone group,the difference in the apoptosis rate at the same time point and the same concentration at different time points were statistically significant,along with the increasing concentration of walnut quinone,the apoptosis rate of oral squamous cell carcinoma Tac8113 showed a significant upward

关 键 词：胡桃醌 口腔鳞癌 Tac8113细胞 增殖 凋亡 细胞周期 

分 类 号：R285.5[医药卫生—中药学]