一株鸭源沙门菌噬菌体的分离鉴定与生物学特性研究  

Isolation,identification and biological characteristics of a Salmonella bacteriophage

作　　者：顾庆林 包涛涛 鲜思美[1,3] 包细明 张友 杨倩 梁倩 郑维豪 GU Qing-lin;BAO Tao-tao;XIAN Si-mei;BAO Xi-ming;ZHANG You;YANG Qian;LIANG Qian;ZHENG Wei-hao(College of Animal Science.Guizhou University,Guiyang 550025;Institute of Animal Disease Research of Guizhou,Guiyang 550025,China;Agriculture and Rural Bureau of Southeast Guizhou,Kaili 556000,China)

机构地区：[1]贵州大学动物科学学院,贵州贵阳550025 [2]贵州省黔东南州农业农村局,贵州贵阳550025 [3]贵州省动物疫病研究所,贵州凯里556000

出　　处：《中国兽医科学》2022年第5期610-616,共7页Chinese Veterinary Science

基　　金：贵州省农业科技攻关项目[黔科合支撑(2016)2506号]。

摘　　要：为了获得鸭源沙门菌噬菌体,本研究以鸭源沙门菌为宿主菌,采用双层琼脂平板法从污水样品中分离纯化得到1株鸭源沙门菌噬菌体,对噬菌体的效价、形态、最佳感染复数(MOI)、酸碱耐受性、热稳定性进行测定,绘制了一步生长曲线,并进行了体外抑菌试验。结果显示,分离到1株鸭源沙门菌噬菌体,将其命名为BpS。该噬菌体能高效裂解鸭源沙门菌菌株,在菌苔表面形成清晰透亮、直径约为1.5 mm的圆形噬菌斑;效价为1.10×10^(10)pfu/mL;电镜观察噬菌体BpS直径约为100 nm,尾部直径约为20 nm,长约125 nm,属于有尾病毒目、肌尾病毒科;最佳感染复数为0.01;在pH为6.0~9.0时具有较高的抵抗力;温度低于50℃时存活率为80%以上;潜伏期和裂解期分别为20 min和70 min;体外抑菌试验表明,BpS可以在3 h内有效抑制沙门菌的生长。结果表明,噬菌体BpS裂解效果较好、特异性强,在生产中具有防治耐药鸭源沙门菌的潜力,有望成为防控耐药沙门菌的抗生素替代产品。In order to obtain Salmonella duck-derived phage,this study used Salmonella duck-derived as the host bacteria.A double-layer agar plate method was used to isolate and purify a Salmonella duck-derived phage from sewage samples.The multiplicity of infection(MOI),acid-base tolerance,thermal stability,one-step growth curve and in vitro antibacterial experiments were determined.The results showed that a Salmonella duck-derived phage was isolated and named BpS.This phage can efficiently lyse the duck-derived Salmonella strain and form a clear and transparent round plaque with a diameter of about 1.5 mm on the surface of the moss,the titer is 1.10×10^(10)pfu/mL.The diameter of the phage BpS is about 100 nm,the diameter of the tail is about 20 nm,and the length is about 125 nm.All have high resistance,the survival rate is more than 80%when the temperature is lower than 50℃,the incubation period and the lysis period are 20 min and 70 min,respectively.In vitro antibacterial experiments show that BpS can effectively inhibit the growth of Salmonella within 3 hours.Bacteriophage BpS has a good lysis effect,strong specificity,and has the potential to prevent and control drug-resistant Salmonella from ducks in production.It is expected to become an antibiotic substitute for the prevention and control of drug-resistant Salmonella.

关 键 词：沙门菌 噬菌体 分离鉴定 生物学特性 

分 类 号：S852.612[农业科学—基础兽医学]