阳离子脂质体体外转染实验条件的优化研究  

Optimization of cationic liposome transfection conditions in vitro

作　　者：李洪梅 姜恩平 裴轶劲 孙艳芹 尹金宝 LI Hongmei;JIANG Enping;PEI Yijin;SUN Yanqin;YIN Jinbao(Department of Pathology,Guangdong Medical University,Dongguan 523808;Department of Physiology,Guangdong Medical University,Dongguan 523808)

机构地区：[1]广东医科大学病理学系,广东东莞523808 [2]广东医科大学生理学教研室,广东东莞523808

出　　处：《当代医药论丛》2022年第13期85-87,共3页

基　　金：广东省医学科学技术研究基金项目(A2020474);广东医科大学自然科学面上培育项目(GDMUM201811)。

摘　　要：阳离子脂质体是被公认的有研发前景的基因传递载体。任何一种商品化的阳离子脂质体转染试剂说明书中给出的转染步骤都是一致的。但应用其转染不同大小的目的基因及用其与不同受体细胞转染相同目的基因时,转染效率均有所不同。用阳离子脂质体法成功转染核酸的最关键一环就是前期转染条件的优化。本文基于阳离子脂质体介导核酸进入细胞的过程,从影响转染效率的六个方面对以往优化转染条件的重要经验和体会加以总结,以期与本领域的同仁分享和探讨。Cationic liposomes are widely recognized as promising gene delivery vectors.The transfection steps given in the instructions of any commercially available cationic liposome transfection agent are consistent.However,the transfection efficiency was different when it was used to transfect different size of target gene and the same target gene with different recipient cells.The most important step for the successful transfection of nucleic acid by cationic liposome is the optimization of the initial transfection conditions.Based on the process of nucleic acid entering cells mediated by cationic liposome,this paper summarizes the important experience of optimizing transfection conditions from six aspects affecting transfection efficiency,in order to share and discuss with colleagues in this field.

关 键 词：阳离子脂质体 核酸 转染 优化 

分 类 号：R34[医药卫生—基础医学]