木犀草素通过CTGF/EGFR通路对人结肠癌细胞LoVo增殖和凋亡的影响  

Effects of luteolin on proliferation and apoptosis of human colon cancer cells LoVo via CTGF/EGFR pathway

作　　者：钟轩 王红钰 高建超 赵金来 张诏然 ZHONG Xuan;WANG Hongyu;GAO Jianchao;ZHAO Jinlai;ZHANG Zhaoran(Gastrointestinal Surgery of Tangshan Central Hospital,Tangshan 063000,Hebei,China;Tumor Surgery of Tangshan Workers Hospital,Tangshan 063000,Hebei,China)

机构地区：[1]唐山中心医院胃肠外科,河北省唐山市063000 [2]唐山市工人医院肿瘤外科,河北省唐山市063000

出　　处：《中南医学科学杂志》2022年第4期486-490,共5页Medical Science Journal of Central South China

基　　金：河北省医学科学研究课题(20191341)。

摘　　要：目的探讨木犀草素通过结缔组织生长因子(CTGF)/表皮生长因子受体(EGFR)通路对人结肠癌细胞LoVo增殖和凋亡的影响。方法MTT法确定木犀草素半数抑制质量浓度、作用时间。将细胞实验分为阴性对照组、阳性对照组、木犀草素低、中、高剂量组。阴性对照组细胞不进行处理,阳性对照组用40μg/L西妥昔单抗干预,木犀草素低、中、高剂量组分别用20、40、80μg/L木犀草素干预。采用流式细胞术检测各组细胞凋亡率,蛋白印迹法检测细胞CTGF、EGFR、蛋白质丝氨酸苏氨酸激酶(Akt)、B细胞淋巴瘤/白血病-2(Bcl-2)、Bcl-2相关X蛋白(Bax)、半胱氨酸天冬氨酸酶3(Caspase-3)蛋白水平。结果MTT法确定木犀草素半数抑制质量浓度40μg/L,半数抑制时间24 h。随着木犀草素质量浓度增加,LoVo细胞增殖率降低(P<0.05)。与阴性对照组比较,其余各组细胞凋亡率、Bax和Caspase-3蛋白均增加,细胞CTGF、p-EGFR/EGFR、Akt和Bcl-2蛋白均降低(P<0.05)。与阳性对照组比较,木犀草素各剂量组细胞凋亡率、Bax和Caspase-3蛋白均降低,细胞CTGF、p-EGFR/EGFR、Akt和Bcl-2蛋白均增加,且随木犀草素剂量增加呈剂量效应关系(P<0.05)。结论木犀草素抑制LoVo细胞增殖,诱导LoVo细胞凋亡,其机制可能与抑制CTGF/EGFR通路的激活有关。Aim To investigate the effect of luteolin on the proliferation and apoptosis of human colon cancer cell LoVo via connective tissue growth factor(CTGF)/epidermal growth factor receptor(EGFR)pathway.Methods MTT method was used to determine the half inhibitory concentration and action time of luteolin.The cell was divided into negative control group,positive control group,low-dose luteolin group,middle-dose luteolin group and high-dose luteolin group.Negative control group was not treated,positive control group was treated with 40μg/L cetuximab,luteolin low-dose,medium-dose and high-dose groups were treated with 20,40,80μg/L luteolin,respectively.The apoptosis rate of each group was detected by flow cytometry,and the protein levels of CTGF,EGFR,Serine threonine kinase(Akt),Bcl-2,Bcl-2 associated X protein(Bax)and cysteine aspartase 3(Caspase-3)were detected by western blotting.Results MTT method was used to determine the median inhibitory concentration of luteolin 40μg/L,24 h as the median inhibitory time.With the increase of luteolin concentration,the proliferation rate of LoVo cells decreased(P<0.05).Compared with negative control group,the apoptosis rate,Bax and Caspase-3 protein levels in the other groups were increased,and the CTGF,p-EGFR/EGFR,Akt and Bcl-2 protein levels decreased(P<0.05).Compared with positive control group,the apoptosis rate,Bax and Caspase-3 protein levels in the all dose luteolin group were decreased,and the levels of CTGF,p-EGFR/EGFR,Akt and Bcl-2 protein increased,and the effect of luteolin dose groups presented a dose-response relationship with the increase of luteolin dose(P<0.05).Conclusion Luteolin can inhibit LoVo cell proliferation and induce LoVo cell apoptosis,which may be related to inhibiting the activation of CTGF/EGFR pathway.

关 键 词：木犀草素 结肠癌 细胞增殖 细胞凋亡 CTGF/EGFR通路 

分 类 号：R285.5[医药卫生—中药学]